影响反胶束体系萃取蛋白能力的因素及机理  

Factors affecting reverse micelle extraction of protein and mechanism

作　　者：赵晓燕[1] 薛文通[2] 陈复生[3] 李里特[2] 

机构地区：[1]清华大学材料科学与工程系,北京100084 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]河南工业大学科研处,河南450052

出　　处：《农业工程学报》2009年第11期354-360,共7页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家教育部科学技术重点资助项目(205094);河南省杰出人才创新基金(0521000500)

摘　　要：该文对影响二-(2-乙基已基)琥珀酸酯磺酸钠(AOT)反胶束萃取蛋白能力的因素与机理进行了研究,可以用来解释AOT/异辛烷反胶束溶液分离萃取蛋白与油脂时,萃取率变化的原因,进一步了解反胶束分离萃取蛋白质分子的机理。试验结果表明随着反胶束体系中黏度增加,微乳液增溶水量即水的物质的量与表面活性剂物质的量之比(W0)增大时,使得反胶束萃取蛋白质与油脂的能力增强,从而使萃取率升高。根据Gibbs吸附公式,得出AOT浓度在0.08g/mL时,反胶束液具有较高的界面活性,W0值也较大,有利于提取蛋白质。利用荧光光谱法和电导法研究AOT反胶束体系的结构,可知W0为11～14时,AOT浓度大于0.06g/mL,反胶束体系较稳定,该体系是由渗透型和非渗透型的结合,从而可以了解反胶束体系的相行为变化。The study was focused on the factors affecting AOT reverse micelle extraction of protein and mechanism, which could explain the reason for the extraction yield changes when extracting and separating protein and oil from AOT reverse micelle, and could further understand the mechanism of separation biomolecules using reverse micelle extraction. The results indicated that the molar ratio of water to surfactant （W0） increased with the increase of the viscosity of reverse micelle, and the capacity of extracting protein and oil from reverse micelle increased. According to Gibbs formula, it was concluded that reverse micelle of 0.08 g/mL AOT showed the higher interface activity and moisture （W0）, which was in favor of the protein and oil extraction in reverse micelle. The structure of reverse micelle was investigated by fluorescence spectra and conductivity methods. The results showed that the reverse micellar system was quite stable at W0=1 1- 14, [AOT] ≥0.06 g/mL, and the AOT reverse micelle system seemed a combination type of a nonpercolation system and a percolation svsterm and the conductivity could reflect the changes of reverse micelle phase.

关 键 词：反胶束 蛋白 黏度 表面张力 增溶水量 电导率 

分 类 号：TS225.44[轻工技术与工程—粮食、油脂及植物蛋白工程]