原料乳中大肠杆菌O157:H7的快速检测  

Rapid detection of Escherichia coli O157:H7 in raw milk

作　　者：姜毓君[1,2] 王明娜 吕琦[4] 姚丽燕[2] 赵凤[2] 崔英俊[2] 

机构地区：[1]国家乳业工程技术研究中心,哈尔滨150086 [2]乳品科学教育部重点实验室东北农业大学食品学院,哈尔滨150030 [3]内蒙古伊利实业集团股份有限公司,呼和浩特101500 [4]中国医学科学院实验动物研究所,北京100021

出　　处：《中国乳品工业》2009年第11期43-44,47,共3页China Dairy Industry

基　　金：国家科技支撑计划(2006BAD04A08;2009BADB9B06);黑龙江省普通高校青年学术骨干支持计划(1151G006)

摘　　要：建立了一种快速检测原料乳中大肠杆菌O157:H7的PCR技术。该方法利用过滤富集菌体后的PCR技术来检测原料乳中大肠杆菌O157:H7,先对人工污染大肠杆菌O157:H7的原料乳进行离心脱脂,然后添加EDTA-2Na获得澄清乳液,最后通过0.45μm微膜过滤收集菌体,整个过程只需6h左右即可完成,检测灵敏度高达101mL-1。这种方法在传统检测方法的基础上做了有效改进,使得原料乳中的大肠杆菌O157:H7的检测能够快速、准确、灵敏的进行。The PCR was developed for detection of Escherichia coli O157：H7 in raw milk. Pathogen was concentrated from raw milk by straightforward filtration and was detected by a rapid and sensitive PCR system for detection of Escherichia coli O157：H7. The raw milk, artificially contaminated Escherichia coli O157：H7, was skimmed by centrifugation and disodium EDTA was added to get a clarified solution. The bacteria were collected with a 0.45 μm membrane filtration and then subjected to the PCR assay. The assay from filtration to PCR can be completed in about 6h and the limit of detection for PCR was as low as 10 cfu of bacteria in 1 mL of raw-milk. The study, which increases the accuracy and sensitivity, improves traditional methods and it can be applied to detection for the foodborne pathogen in raw milk.

关 键 词：PCR 原料乳 大肠杆菌O157:H7 过滤 快速检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]