造纸污泥同步糖化发酵产乙醇的研究  

Investigation on the Production of Ethanol from Paper Sludge by Simultaneous Saccharification and Fermentation

作　　者：江丹[1] 李旭晖[1] 朱明军[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《食品与发酵工业》2009年第11期32-35,共4页Food and Fermentation Industries

摘　　要：对2种造纸污泥(脱墨废纸污泥PS1和化学制浆污泥PS2)利用商业纤维素酶和酒精酵母SHY08-3同步糖化发酵(SSF)产乙醇的工艺条件进行了研究。结果表明,化学制浆污泥具有较好的可酶解发酵性能,而脱墨污泥对酶解具有一定的抗性。在底物浓度(以纤维素计)35 g/L、纤维素酶添加量55FPU/g有效纤维素浓度(effective cellulose,EC)、接种量6%、反应温度37℃和摇床转速200 r/min条件下发酵72 h,乙醇发酵效率达最大值,分别为72.71%和95.97%。研究结果证明,利用造纸污泥同步糖化发酵生产乙醇具有潜在的应用前景。A study was undergone to evaluate the role of some fermentation parameters like substrate concentration, enzyme loading, inoculum size, temperature and rotation speed on ethanol production from deinking paper sludge （PS1） and chemical pulping sludge （PS2） by simultaneous saccharification and fermentation using cellulase and Saccharomyces cerevisiae SHY08 -3. The resuh showed that chemical pulping sludge had good enzymability and fermentability, whereas deinking paper sludge was not susceptible to cellulase. The maximum ethanol production efficiency of PS1 and PS2, 72.71% and 95.97% respectively, were reached at 72h of fermentation under the conditions of substrate concentration of 35g/L （according to cellulose）, cellulase loading of 55FPU/gEC （effective cellulose）,inoculum size of 6% , temperature of 37~C and rotation speed of 200 r/min. The results above effectively elucidated that paper sludge has good potential application for ethanol production using simultaneous saecharification and fermentation process.

关 键 词：造纸污泥 酒精酵母 同步糖化发酵 乙醇 纤维素酶 

分 类 号：TS262.2[轻工技术与工程—发酵工程] TQ223.122[轻工技术与工程—食品科学与工程]