L-乳酸调控乳酸产生菌产物光学纯度的分析  

Effects of cultivation conditions on the optical purity of L(+)-lactic acid

作　　者：孟武[1] 李十中[2] 封文涛[1] 张晗星[2] 王瑞明[1] 

机构地区：[1]山东轻工业学院食品与生物工程学院,济南250353 [2]清华大学核能与新能源研究院,北京100084

出　　处：《生物工程学报》2009年第11期1679-1683,共5页Chinese Journal of Biotechnology

基　　金："十一五"国家科技支撑计划(No.2006BAD07A00)资助~~

摘　　要：发酵初期在米根霉菌发酵培养基中添加L-乳酸可以调控发酵产物乳酸的光学纯度。随着L-乳酸添加量的增加,所产L-乳酸的光学纯度随之增加,当L-乳酸的添加量≥1.5g/L时,D-乳酸不再产生。同时,L-乳酸的产量、生物量、糖转化率也随之降低。该调控方法对乳酸菌调控产L-乳酸光学纯度影响不大,对大肠杆菌发酵调控产D-乳酸光学纯度没有效果。The effect of cultivation conditions on the optical purity of L（＋）-lactic acid produced by Rhizopus oryzae HZS6 was investigated.The isomeric composition of lactic acid was influenced by the supplementation of L（＋）-lactic acid to fermentation medium.L（＋）-isomer increased with the dosage,no D（-）-lactic acid was observed when the concentration of supplemented L（＋）-lactic acid in matrix was ≥1.5 g/L.However,the L（＋）-lactic acid yield,biomass and glucose conversion rate decreased with the dosage.With the same method,the supplementation of L（＋）-lactic to substrate had no influence on isomeric composition of lactic acid by Lactobacillus and Escherichia coli.

关 键 词：L-乳酸 D-乳酸 光学纯度 米根霉 乳酸菌 大肠杆菌 调控 

分 类 号：R318.08[医药卫生—生物医学工程]