L-谷酰胺产生菌株的选育和发酵条件的研究  

STUDIES ON THE SELECTIVE BREEDING AND FERMENTATION CONDITIONS OF L GLUTAMINE PRODUCER

作　　者：马柯[1] 徐建华[1] 曹晖[1] 岳峡 傅筱冲[1] 

机构地区：[1]江西省科学院微生物研究所

出　　处：《江西科学》1998年第4期242-248,共7页Jiangxi Science

摘　　要：报道了Ｌ谷酰胺产生菌Ｂｃ－６６诱变育种及其摇瓶发酵条件研究的结果。以黄色短杆菌ＡＴＣＣ１４０６７为出发菌种，经逐级诱变处理，选育１株能高产Ｌ谷酰胺，副产物较少，且对磺胺胍具抗性的变异株Ｂｃ－６６。在适宜的培养条件下，摇瓶发酵７２ｈ，可积累谷酰胺达３１～３３ｍｇ·ｍＬ－１，且遗传稳定性较好。对该菌株的发酵条件作了较系统的试验，摸索出较以前简化的培养基，有利于今后的进一步研究。The selective breeding of an L glutamine producer and its fermentative conditions in shaking flask were studied.Mutant Bc 66 was derived from Brevibacterium flavum ATCC14067 by means of mutagenesis with diethyl sulphate(DES).The mutant showed a good character.Such as higher product of L glutamine,less by products and good resistance to sulfaguanidine(SG).It can accumulate 31～33 mg·mL -1 L glutamine after 72 hrs in shaking flask culture under optimum conditions.The fermentative medium and conditions were also discussed in this paper.

关 键 词：谷酰胺 黄色短杆菌 育种 发酵 菌株 

分 类 号：TQ922.9[轻工技术与工程—发酵工程] TQ464.7