莲子壳提取物中黄酮类物质的抗氧化活性及结构初探  

Antioxidant Activity and Structure of Flavonoids from Epicarp of Nelumbo nucifera Gaertn.

作　　者：黄迪惠[1] 胡崇琳[1] Husam M Credy 谢笔钧[1] 何慧[1] 杨尔宁[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2009年第23期209-213,共5页Food Science

摘　　要：目的:研究莲子壳中的黄酮类化学成分及抗氧化活性。方法:莲子壳的60%乙醇提取物经过大孔树脂初步分离之后,采用Toyopearl HW-40S凝胶柱进一步分离得到了6个组分(LF-F1～LF-F6),再采用高效液相以及液质联用系统对其中的组分2(LF-F2)组成及结构进行分析,并采用化学发光法等方法研究了LF-F2的抗氧化活性。结果:莲子壳黄酮粗提物中总黄酮为94.1%;LF-F2中黄酮甙元是由槲皮素、杨梅黄酮、山奈酚、异鼠李素等组成的。LF-F2对超氧阴离子自由基、羟自由基以及对双氧水的清除作用的IC50分别为420、40、0.6μg/ml。结论:从莲子壳中鉴定出LF-F2中有芦丁存在。LF-F2具有很好的抗氧化效果,并存在剂量效应关系。This study was aiming to study the chemical composition of flavonoids of ethanolic extract of Epicarp of Nelumbo nucifera Gaertn as well as its antioxidant activity. The ethaonolic extract of lotus Epicarp was isolated using macroporous resin and Toyopearl HW-40S gel column to acquired 6 compounds （LF-F1～LF-F6）. The composition and structure of LF-F2 was analyzed by HPLC and LC/MS and the antioxidant activity was measured by chemiluminescence. Results showed that total flavonoids accounts for 94.1% of the extracts （60% ethanol） of lotus Epicarp. Flavonols of LF-F2 comprised quercetin, myricetin, kaempferol, isorhamnetin and so on. IC50 for LF-F2 to superoxide anion radical, hydroxyl radical and hydrogen peroxide were 420, 40, 0.6μg/ml, respectively. The existence of rutin in LF-F2 was identified for the first time. Fraction LF-F2 possessed good antioxidant activity in a dose dependent manner.

关 键 词：黄酮 分离纯化 抗氧化活性 化学发光 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]