沙棘叶多糖的提取工艺及抗氧化作用的研究  

Study of extraction and antioxidant of polysaccharide from Hippohae rhamnoides L. leave

作　　者：包怡红[1] 秦蕾[1] 王戈 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]中共黑龙江省委办公厅,黑龙江哈尔滨150007

出　　处：《食品工业科技》2010年第1期286-290,共5页Science and Technology of Food Industry

摘　　要：以沙棘叶多糖得率为指标,通过单因素及正交实验,对水提、微波辅助及超声波辅助三种提取方法进行比较,超声波辅助法的最佳提取条件为:液固比60∶1,超声波功率700W,超声波作用时间40min。在此条件下,超声波法的最佳提取率为7.50%,高于微波法的最佳提取率7.05%和水提法的最佳提取率6.24%。实验结果表明:沙棘叶多糖具有一定的体外抗氧化能力,其中当多糖浓度为1200μg/mL时,对O-2.的清除率比同浓度的VC还高18.92%。Taking the rate of polysaccharide from Hippohae rhamnoides L.leaves as evaluation index, and through single factor and orthogonal tests, the three methods of extraction ： water, microwave- assisted and ultrasonic- assisted,were compared.The results showed the best ultrasonic-assisted extraction rate of 7.50% ,higher than the microwave method the best rate of 7.05% and water methods the best rate of 6.24%. The best conditions of ultrasonic extract methods were as follows： liquid-solid ratio 60： 1, the ultrasonic power 700W, ultrasonic time 40min.The polysaccharide extracted from Hippohae rhamnoides Lleave had certain degree antioxidant capacities in vitro, for example,when the concentration of polysaccharide was 1200pg/mL, the removal effect towards O2- was of higher than Vc by 18.92% .

关 键 词：提取 多糖 沙棘叶 体外抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]