碱性果胶酶的生物制造及其在纺织工业清洁生产中的应用研究进展  

Advances in microbial production of alkaline polygalacturonate lyase and its application in clean production of textile industry

作　　者：刘龙[1,2] 汪志浩[1,2] 张东旭[1,2] 李江华[1,2] 堵国成[1,2] 陈坚[2,3] 

机构地区：[1]江南大学生物工程学院,无锡214122 [2]江南大学工业生物技术教育部重点实验室,无锡214122 [3]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《生物工程学报》2009年第12期1819-1828,共10页Chinese Journal of Biotechnology

基　　金：国家杰出青年基金(No.20625619);教育部新世纪优秀人才支持计划(No.NCET-05-0488);863项目(No.2009AA02Z204);江苏省科技支撑计划(No.SBE200900022);国家自然基金重点项目(No.20836003);江南大学自主科研计划(No.JUSRP30901)资助~~

摘　　要：以下综述了碱性果胶酶的生物制造及其在纺织工业清洁生产中的应用研究进展。微生物发酵法是目前生产碱性果胶酶的主要方式,枯草芽孢杆菌是碱性果胶酶工业发酵生产中效果较好的野生菌株。影响发酵法生产碱性果胶酶的主要因素有:底物浓度及其流加方式、细胞浓度、搅拌转速、通气速率、pH、温度等。构建基因工程菌为碱性果胶酶的发酵生产开辟了一条有效途径,其中重组毕赤酵母的产酶水平最高,在10吨发酵罐上酶活达1305U/mL。碱性果胶酶可用于棉织物前处理的精练工艺,与传统高温碱煮相比,具有保护纤维、提高精练效率、降低能耗和污染的优势。通过分子定向进化技术对碱性果胶酶进行分子改造,使其催化特性更加适合于纺织精练工艺,进而实现纺织工业的清洁生产是未来的研究重点和热点。We reviewed the microbial production of alkaline polygalacturonate lyase （PGL） and its application in the clean production of textile industry.Currently PGL is mainly produced by microbial fermentation and Bacillus sp.is an ideal wild strain for PGL production.Microbial PGL production was affected by many factors including the concentration and feeding mode of substrate,cell concentration,agitation speed,aeration rate,pH and temperature.Constructing the recombinant strain provided an effective alternative for PGL production, and the concentration of PGL produced by the recombinant Pichia pastoris reached 1305 U/mL in 10 m^3 fermentor. The recombinant Pichia pastoris had the potential to reach the industrial production of PGL. PGL can be applied in bio-scouring process in the pre-treatment of cotton. Compared with the traditional alkaline cooking process, the application of PGL can protect fiber, improve the bio-scouring efficiency, decrease energy consumption and alleviate the environmental pollution. The future research focus will be the molecular directed evolution of PGL to make PGL more suitable for the application of PGL in bio-scouring process to realize the clean production of textile industry.

关 键 词：碱性果胶酶 发酵法生产 枯草芽孢杆菌 重组毕赤酵母 生物精练 

分 类 号：TS192.5[轻工技术与工程—纺织化学与染整工程]