油菜蜂花粉醇提物中抑菌活性物质的研究  

Antibacterial Activity of Bioactive Components in Ethanol Extract from Rape Bee Pollen

作　　者：孙丽萍[1] 徐响[1] 丁小宇[2] 李春艳[2] 安仲姚[2] 董捷[1] 张红城[1] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]东北农业大学资源与环境学院,黑龙江哈尔滨150030

出　　处：《食品科学》2010年第1期48-50,共3页Food Science

基　　金：国家公益性行业(农业)科研专项经费项目(nyhyzx07-041);农业部现代农业蜂产业技术体系建设专项资金项目(NYCYTX-43)

摘　　要：本实验选用我国产量最高的油菜蜂花粉为原料,采用牛津杯法测定油菜蜂花粉乙醇提取物中甲酸提取组分对常见食品污染微生物的抑制效果。结果表明:0.5%甲酸提取组分具有较强的抑菌作用,对大肠埃希氏菌、金黄色葡萄球菌、铜绿假单胞菌和枯草芽孢杆菌的最小抑菌浓度(MIC)分别为25、50、50、100mg/mL;经HPLC分析表明乙醇提取物中甲酸提取组分抑菌有效成分可能为弱极性物质。Antibacterial activity of rape bee pollen extract was investigated using Oxford cup method.Results indicated that 0.5% formic acid extraction fraction from ethanol extract of rape bee pollen exhibited strong antibacterial activity.Minimum inhibitory concentrations（MIC） of formic acid extraction fraction against Escherichia coli,Staphylococcus aureus,Pseudomonas aeruginosa and Bacillus subtilis were 25,50,50 mg/mL and 100 mg/mL,respectively.HPLC analysis showed that bioactive components with antibacterial activity in formic acid elution fraction were weakly polar substances.

关 键 词：油菜蜂花粉 抑菌活性 醇提物 

分 类 号：S896.3[农业科学—特种经济动物饲养]