HPLC-DAD分析红茶儿茶素类物质和咖啡因的方法研究  

A fast and sensitive HPLC-DAD method for the determination of catechin and caffeine in black tea

作　　者：谷勋刚[1] 蔡继宝[2] 张正竹[1] 苏庆德[2] 

机构地区：[1]安徽农业大学茶叶生化与生物技术农业部重点实验室,合肥230036 [2]中国科学技术大学化学系,合肥230026

出　　处：《安徽农业大学学报》2010年第1期5-10,共6页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(20405013);国家科技支撑项目(2007BAD58B04);中国博士后基金项目(20080430757)共同资助

摘　　要：建立一种梯度洗脱的儿茶素类和咖啡因HPLC—DAD灵敏、快速的定量方法。确定最佳波长为205nm，5种儿茶素（EGCG，EGC，ECG，EC，＋C）和咖啡因的检测限（LOD）达到0．01～0．04g·mL^-1。采用反向C18（150×4．6mm，ID；5μm）柱分离，以乙腈和0．03％三氟乙酸溶液为流动相梯度洗脱，在1mL·min^-1的流速下，整个色谱运行过程仅需15rain目标物质的回收率在92％-110％范围，相对标准偏差小于2．7％。该方法稳定性好、专一性强，适合红茶儿茶素类和咖啡因的准确定量分析。A gradient elution HPLC-DAD method has been developed for sensitive and fast determination of five main c atechins, namely epigallocatechin gallate （EGCG）, epigalloeatechin （EGC）, epicatechin gallate （EGG）, epicatechin （EC）, and catechin （ ＋ C）, and caffeine in teas. The optimial wavelength was found at 205 nm, where limit of detections （LOD） for catechins and caffeine obtained were in the range of 0. 01 -0. 04 μg.mL^-1. Reverse-phase C18 column （150×4.6 mm ID,5 μm particle size;Agilent） and gradient elution consisted of acetonitrile and 0. 03% trifluoroacetic acid solution （v/v） were employed for HPLC separation, in which, at 1 mL·min^-1 of flow-rate, the extract was determined within 15 min. Recoveries were in range of 92% -110% ,and repeatability expressed as RSD was less than 2.7%. Because of stability and specialty, the developed technique can be used as an ideal analytical method for determination of five catechins and caffeine in black tea.

关 键 词：高效液相色谱 儿茶素类 咖啡因 红茶 灵敏度 

分 类 号：S571.1[农业科学—茶叶生产加工] TS201.2[农业科学—作物学]