带鱼下脚料水解螯合物制备及其生物特性研究  

Studies on the Functionality of Protein Hydrolysates of Chelate by Enzymolysis from Hairtail Waste

作　　者：陈国章 谢超[2] 

机构地区：[1]浙江兴业集团有限公司,浙江舟山316100 [2]浙江海洋学院食品与药学学院、医学院,浙江舟山316004

出　　处：《浙江海洋学院学报（自然科学版）》2010年第1期49-54,共6页Journal of Zhejiang Ocean University(Natural Science Edition)

基　　金：浙江省重大科技专项(2007C12013)

摘　　要：通过对带鱼下脚料水解物亚铁螯合修饰及其功能性研究,为带鱼下脚料水解物的高效利用奠定理论基础。采用复合酶水解法和亚铁修饰法进行初步研究。未脱脂的带鱼下脚料水解螯合修饰的最适条件为蛋白质水解度为5%、螯合pH为7.0、螯合温度为20℃、螯合时间为15min;经无水乙醇沉淀可获得4种螯合组分,分别为:CA,螯合后产生的沉淀物组分;CB,沉淀于50%无水乙醇中的组分;CC,悬浮于80%无水乙醇中组分;CD,沉淀于80%无水乙醇底部组分。在这4种组分中,CB抗氧化活性最高,其抗氧化活性可达到a-生育酚活性的92%;CD抗菌性最强,它对大肠杆菌、葡萄球菌、沙门氏菌以及枯草芽胞杆菌都具有抗菌性。带鱼下脚料水解螯合可作为食品添加剂在食品工业中应用。This article will provide theoretical basis for the high-efficient utilization of hairtail hydrolysis by product chelates by researching the function of protein hydrolysates of chelate by enzymolysis from hairtail waste. Complex enzymolysis and Fe^2＋-chelate methods were used in this paper. The optimum formation conditions of the hydrolysate chelating Fe^2＋ are 5% of DH, 7.0 of pH, 20 ℃ of temperature and 15 min of chelating time for FM （material not being defatted）. Four types of chelating Fe^2＋ including CA （deposit after chelating）, CB （deposit in 50% of absolute ethanol solution）, CC（suspended deposit in 80% of absolute ethanol solution） and CD （bottom deposit in 80% of absolute ethanol solution） were obtained through fractional precipitation with absolute ethanol from FM. Among all of the chelates, the CB possesses the most effective antioxidative activity and the activity is 92% as high as that of a-tocopherol; and only CD possesses the most effective antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella typhi and Bacillus subtilis. Protein hydrolysates of Fe-FPH from hairtail waste can be used for food industry.

关 键 词：带鱼下脚料 复合蛋白酶 螯合 功能性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]