茉莉花茶香气的SPME/GC-MS检测方法  

Detection of Aroma from Jasmine Scented Tea by Using SPME/GC-MS

作　　者：杨江帆[1,2] 杨广[3] 梁小虾[3] 叶乃兴[2] 郑乃辉[4] 林河通[2,5] 

机构地区：[1]武夷学院,福建武夷山354300 [2]福建农林大学茶叶科技与经济研究所,福建福州350002 [3]福建农林大学应用生态研究所,福建福州350002 [4]福建省农业科学院茶叶研究所,福建福安355015 [5]福建农林大学食品科学学院,福建福州350002

出　　处：《热带作物学报》2009年第11期1698-1705,共8页Chinese Journal of Tropical Crops

基　　金：福建省科技厅重大农业科技项目(No.2003S007)资助

摘　　要：为建立茉莉花茶香气的固相微萃取/气相色谱-质谱(SPME/GC-MS)联用的快速检验方法,以"春毫茉莉花茶"为材料,研究萃取头类型、吸附温度、吸附时间和样品量对SPME吸附茉莉花茶香气的影响,同时研究脱附时间对SPME中茉莉花茶香气脱附效果的影响。结果表明:应用SPME/GC-MS技术检测茉莉花茶香气,具有简便、快速、准确和灵敏度高等特点;测定茉莉花茶香气的推荐条件为用150g茉莉花茶样品量、PDMS-100μm萃取头、在60℃下吸附2h、脱附时间5min。In order to establish a rapid detection method for the aroma from jasmine scented tea based on SPME-GC-MS, jasmine scented tea ＇Chunhao＇ was used as experimental material to explore the effects of solid-phase micro-extraction （SPME） types, absorbing time, absorbing temperature, and sample amount of jasmine scented tea on absorption of aroma of jasmine scented tea by SPME, and the effects of desorbing time on desorbing of aroma of jasmine scented tea by SPME. The results showed that this detection method for the aroma of jasmine scented tea by SPME/GC-MS was characteristic of easy operation, rapidness, high precision and high sensitivity. It can be seen from the results that when the aroma of jasmine scented tea was detected by SPME/GC-MS, fused-silica fiber with 100 μm polydimethyl siloxane （PDMS-100 μm） was recommended to be used under the conditions that the sample amount of jasmine scented tea was 150 g at the absorbing temperature of 60 ℃ for 2 hours of absorbing time and 5 minutes of desorbing time.

关 键 词：固相微萃取(SPME) 气相色谱/质谱联用(GC-MS) 茉莉花茶 香气 检测方法 

分 类 号：S571.1[农业科学—茶叶生产加工]