油菜蜂花粉黄酮醇的测定及其抗氧化活性研究  

Hydrolyzed Rape Bee Pollen Ethanol Extract: Qualitative and Quantitative Analysis of Flavonol and Antioxidant Activity Evaluation

作　　者：杨佳林[1] 孙丽萍[1] 徐响[1] 朱青[2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]北京市卫生局临床药学研究所,北京100035

出　　处：《食品科学》2010年第3期79-82,共4页Food Science

基　　金：国家公益性行业(农业)科研专项经费项目(nyhyzx07-041)

摘　　要：本实验通过盐酸酸解蜂花粉乙醇提取物,薄层色谱法分离酸解液中黄酮醇,并测定酸解液中黄酮醇的含量及其抗氧化活性。结果表明:蜂花粉乙醇提取物酸解后,槲皮素(Rf值0.59)含量为0.40mg/g,山奈酚(Rf值0.71)含量为3.98mg/g,酸解液DPPH.清除率强于花粉乙醇提取物。经制备液相分离,采用核磁和质谱鉴定出一种黄酮醇苷,其结构为山奈酚3-O-(2"-O-β-D-吡喃葡萄糖)-β-D-葡萄糖。采用TLC-DPPH技术对蜂花粉乙醇提取物及其酸解液中抗氧化成分进行追踪,为花粉中黄酮醇苷的抗氧性研究提供参考。Ethanol（80% concentration） extract of rape pollen was hydrolyzed with hydrochloric acid and the resulting hydrolysate was analyzed for the qualification and quantitation of flavonol by thin layer chromatography（TLC）,UV-Vis spectroscopy and HPLC,and was subjected to antioxidant activity evaluation.TLC results showed that bands with 0.59 and 0.71 of Rf value were quercetin and kaempferol,respectively.The contents of quercetin and kaempferol in the hydrolysate were 0.40 mg/g and 3.98 mg/g,respectively.The scavenging capacity of rape pollen ethanol extract against DPPH free radicals was poorer than that of its hydrolysate.Preparative high performance liquid chromatography was used to separate a flavonol,which was identified as kaempferol 3-O-（2＂-O-β-D-glucopyranosyl）-β-D-glucopyranoside by MS and NMR.In this work,TLC-DPPH was used to track the antioxidant activity of flavonol in rape pollen,which will provide useful references for further studying the antioxidant activity of active compounds in rape pollen.

关 键 词：油菜蜂花粉 黄酮醇 抗氧化活性 

分 类 号：S896.4[农业科学—特种经济动物饲养]