纳豆芽孢杆菌发酵米糠上清液的整肠作用研究  

Regulatory Effect of Supernatant of Bacillus natto-fermented Rice Bran on Intestinal Tract System in Mice

作　　者：祁红兵[1] 陈钧[2] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000 [2]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品科学》2010年第3期253-257,共5页Food Science

基　　金：河南省信阳师范学院青年骨干教师资助计划项目(2006-014)

摘　　要：目的:为深度开发米糠资源,考察纳豆芽孢杆菌发酵的米糠提取物(supernatant of Bacillus natto-fermentedrice bran,SBRB)的整肠作用。方法:米糠提取物与双歧杆菌的体外共培养试验;灌胃SBRB,检测小鼠肠道正常菌群的生长。结果:体外试验证明纳豆菌在严格厌氧环境不能促进双歧杆菌的生长。加入SBRB与米糠水提液(aqueous extract of rice bran,ABRB)均能显著促进双歧杆菌的生长,提高比率分别为65.2%和17.8%;SBRB经过胃蛋白酶和胰蛋白酶消化后,仍有51.6%的促生率。作为外源因子的SBRB与AERB中含有多种促生成分。动物实验结果表明,灌胃SBRB能够显著促进以厌氧菌为优势菌群的小鼠肠道菌群的生长,抑制好氧菌的增殖,作用效果与微生态制剂培菲康相当;纳豆菌和发酵成分均对小鼠的肠道菌群具有调节作用,两者复合能发挥更强的功能。结论:SBRB具有调整肠道菌群的功能,适于制成合生素制剂。Objective： To exploit the resource of rice bran and investigate the regulation role of supernatant of Bacillus natto-fermented rice bran（SBRB） for intestinal tract system in mice.Methods： The culture supernatant of Bacillus natto in rice bran fermentation medium was co-cultured with Bacillus bifidus.Mice were treated with SBRB via a gavage tube and the quantity of normal flora of intestinal tract in mice was detected.Results： Individual Bacillus natto could not promote the growth of Bacillus bifidus under anaerobic environment in vitro.However,both SBRB and aqueous extract of rice bran（AERB） could promote the growth of Bacillus bifidus by 65.2% and 17.8%,respectively.After enzymatic digestion by pepsin and trypsin,51.6% promotion capacity of SBRB was remained.Similarly,the growth promotion of anaerobic bacteria and growth inhibition of aerobic bacteria were also observed in the intestinal tract in mice treated with SBRB.Individual application of Bacillus natto and its fermentation broth exhibited a regulatory effect on normal flora of intestinal tract in mice.In additions,a synergistic effect of combinatorial application on normal flora of intestinal tract in mice was also observed.Conclusion： SBRB has the function for regulating intestinal tract flora and has a promising potential to be used as synbiotic preparation.

关 键 词：纳豆芽孢杆菌 米糠 整肠 合生素 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]