凉粉草多糖提取及纯化工艺的研究  

Studies on Extracting and Purifying Technologies of Mesona Blumes Polysaccharide

作　　者：冯涛[1] 郁晶晶[1] 杨晓波[1] 

机构地区：[1]上海应用技术学院香料香精技术与工程学院,上海200235

出　　处：《食品工业》2010年第1期82-85,共4页The Food Industry

基　　金：上海市教委科技创新项目(08YZ164)

摘　　要：研究Na2CO3浓度、料液比、提取时间对多糖提取率的影响,双氧水浓度和加入量对多糖脱色效果的影响,比较截留分子量为10K与50K中空纤维膜管对多糖超滤纯化的效果。结果发现,凉粉草多糖的最佳提取工艺为:碳酸钠溶液1.5%(M/V),料液比9/250(M/V),提取1.5h。在此条件下,测得凉粉草多糖的提取率为41.00%,总糖含量为3.495%;100mL料液中加入10%的双氧水20mL时脱色效果可达超滤工艺要求;截留分子量为10K的中空纤维膜管在超滤后多糖的保留率高于50K的。因此可选择截留分子量10K的中空纤维膜管对凉粉草多糖进行纯化。This paper, firstly CNa2CO3, Mesona Blumes： solvent （M/V） and extracting time were investigated on the extraction rate of MBP, then MBP solution was decolorized by H2O2, finally under the pressure of 0.1 MPa, the decolorized MBP solution had been circularly ultra filtered by hollow fiber membrane tube with Mw cutoff of 10 K and 50 K for 2 h respectively. It was concluded that optimal extracting technologies of MBP were 1.5% Na2CO3, Mesona Blumes ： solvent 9/250 （M/V）, and extracting 1.5 h at 95℃ water bath. Under such a condition, extraction rate of MBP was 41.00% and total sugar content was 3.495%. MBG solution was decolorized by 10%, 20 mL H202 added into per 100 mL MBG solution. After ultrafiltration, each of their retention part was precipitated by 70% alcohol. Each MBG obtained was analyzed by determining polysaccharide content, Mw distribution and monosaccharide composition; it was founded that hollow fiber membrane tube with Mw cutoff of l0 K could be better than that of 50 K. So the hollow fiber membrane tube with Mw cutoff of 10 K should be chosen for a further study.

关 键 词：凉粉草 粗多糖 脱色 超滤 纯化 

分 类 号：TQ461[化学工程—制药化工]