纤维素酶水解稻草纸浆制取乙醇的研究  

Fermentative production of ethanol from straw paper pulp by enzymatic hydrolysate

作　　者：杨洋[1] 张玉苍[1] 孙岩峰[1] 何连芳[1] 周长俊[1] 

机构地区：[1]大连工业大学资源开发利用研究所,辽宁大连116034

出　　处：《中国酿造》2010年第2期33-37,共5页China Brewing

基　　金：辽宁省科技厅计划项目(2009223003);2007辽宁省教育厅创新团队项目计划(2007T008);辽宁省高校优秀人才支持计划项目(2008RC08);辽宁省自然科学基金(20072163)

摘　　要：对Candida shehatae发酵稻草纸浆水解液生产乙醇的工艺进行了初步研究。分别对影响酶水解和发酵阶段的各因素进行了优化。结果表明,利用正交试验确定的最佳酶水解条件为:酶用量150U/g料,底物浓度2%,反应温度55℃,pH4.8,反应时间8h,酶解得率可达到73.20%。初始葡萄糖浓度为60g/L,装液量100mL/250mL,无机氮源为(NH)42SO4,转速为100r/min,温度为28℃,pH值为5.5,发酵时间为48h,在此条件下乙醇得率可达49.72%,能达到理论得率的97%,转化率最高为0.36g/g(乙醇/稻草纸浆)。Ethanol production from straw paper pulp hydrolysated by Candida shehatae was investigated in this paper. The conditions that impact the enzyme hydrolysis and fermentation were optimized, respectively. The optimal hydrolysis conditions were obtained through orthogonal test as followed： enzyme concentration 150 U/g material, substrate concentration 2%, temperature 55℃, pH 4.8 and hydrolysis time 20 h. Under these condtions, the yield of reducing sugar hydrolysis is 73.2% ;The optimal conditions of ethanol fermentation were as followed： initial glucose concentration 60 g/L, volumes of shaking flask 100 ml/250 ml, inorganic nitrogen source （NH4）2SO4, rotation speed 100 r/min, pH 5.5, fermentation time 48 h. Under these conditions, the ethanol yield rate reached 49.72%, which was 97.0% of the ethanol theoretical yield. The high conversion was 0.36 g/g （ethanol/srraw paper pulp）.

关 键 词：稻草纸浆 酶水解 发酵 乙醇 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]