一株高产碱性内切聚半乳糖醛酸酶生产菌的筛选与鉴定  

Screening and Identification of a Bacterium with High Yield of Alkaline Endopolygalacturonase

作　　者：李祖明[1,2,3] 张洪勋[2] 薛文通[3] 张保国[2] 李鸿玉[4] 张晓岚[5] 

机构地区：[1]北京联合大学应用文理学院,北京100191 [2]中国科学院生态环境研究中心,北京100085 [3]中国农业大学食品科学与营养工程学院,北京100083 [4]北京联合大学师范学院,北京100011 [5]玉门油田分公司炼油化工总厂,玉门735200

出　　处：《生物技术通报》2010年第2期178-183,共6页Biotechnology Bulletin

基　　金：北京市教委科研计划项目(KM200811417006);北京市属市管高校人才强教计划资助项目

摘　　要：从碱性土样中经分离筛选得到1株固态发酵产碱性内切聚半乳糖醛酸酶活力较高的菌株。经提酶条件优化得到较优的酶液提取提条件为30倍Na2CO3/NaHCO3缓冲液提取1 h。从形态特征、培养特征、生理生化特征以及分子生物学鉴定结果来看,该菌株属B.gibsonii。A bacterial strain highly producing alkaline endopolygalacturonase by solid-state fermentation was obtained from a sample of alkaline soil. The recovery ratio of the enzyme from the isolate was the highest after 1 h of extraction in 30 volume of Na2CO3/ NaHCO3 buffer at 30℃. The isolate was confirmed to be a strain of B. gibsonii after identified by colony characteristics, morphological characteristics,physiologica and biochemical characteristics and molecular biology methods.

关 键 词：碱性内切聚半乳糖醛酸酶 筛选 固态发酵 提取条件优化 鉴定 

分 类 号：TQ925[轻工技术与工程—发酵工程]