HPLC法测定酯化红曲霉发酵液中桔霉素含量  

Determination of Citrinin Content in Monascus rubber Fermenting Liquid by HPLC

作　　者：钱志伟[1] 杨丹丹[1] 徐前景[1] 方尚玲[1] 陈茂彬[1] 

机构地区：[1]湖北工业大学生物工程学院发酵工程省部共建教育部重点实验室,湖北武汉430068

出　　处：《酿酒科技》2010年第3期107-109,共3页Liquor-Making Science & Technology

基　　金：湖北省自然科学基金项目(2008CDB063);发酵工程省部共建教育部重点实验室项目

摘　　要：研究了酯化红曲霉发酵液中桔霉素含量测定的条件和方法。采用色谱柱ZORBAX Eclipse XDB C18(5μm,250 mm×4.6 mm);乙腈∶水(磷酸调pH2.5)为50∶50(v∶v)为流动相;柱温28℃;流速1 mL/min,于波长λex331nm、λem 500 nm荧光检测器检测,采用外标法定性、定量。在此色谱条件下,桔霉素呈现较好的分离效果和重现性,其保留时间为6.454 min左右,最低检测浓度为32μg/L,平均回收率为98.95%,RSD=1.53%。The determination conditions of citrinin content in Monascus rubber fermenting liquid were studied and summed up as follows： a ZOR- BAX Eclipse XDB C18 culumn（5 um,250 mm×4.6 mm） used, acetonitrile：water（pH2.5）-50：50（v/v）as mobile phase, the column temperature was at 28 ℃ the flow rate was 1.0 mL/min, then citrinin content was detected by a fluorescence detector at λex=33 lnm and λem=500 nm, and external standard method was adopted for qualitative and quantitative determination. Under the above conditions, eitrinin showed good separation and reproducibility, the retention time was about 6.454 min,the minimum determination limit was 32 ug/L, the average recovery rate was 98.95 %,and RSD was 1.53 %.

关 键 词：检测分析 高效液相色谱法 红曲霉 发酵液 桔霉素 

分 类 号：TS202.3[轻工技术与工程—食品科学]