马铃薯淀粉水解液对茁霉多糖发酵的影响  

Effect of potato syrup on the fermentation conditions of pullulan

作　　者：王虹蕴[1] 张盛贵[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2010年第1期125-129,共5页Journal of Gansu Agricultural University

基　　金：甘肃生物技术专项基金(GNSW-2005-06)

摘　　要：以马铃薯为原料,采用双酶法制备的水解液为碳源,对出芽短梗霉D1-11的多糖发酵工艺进行了筛选.通过碳源和二价阳离子的单因素试验发现,经β-淀粉酶制备的水解液较SNase303糖化酶制备的水解液更适合做茁霉多糖的发酵碳源,且脱盐脱色处理会提高多糖的产率.DE值50%的β-淀粉酶糖化液经脱色脱盐处理,在水解液碳源浓度80g.L-1时多糖产量最高,为21.98g.L-1,CaCO3在0.1g.L-1时多糖产量最高,为21.11g.L-1;均匀试验确定的摇瓶培养最佳发酵工艺为:CaCO30.06g.L-1,碳源82.25g.L-1,装液量26.62%,接种量11.99%,pH7.5,茁霉多糖产量可达28.90g.L-1.Potato syrup was used as the material,which was produced by the double-enzyme method as the carbon source of mutant strain Aureobasidium pullulans D1-11.The single factor test was involved in the carbon source and divalent cation.The results showed that the syrup produced with β-amylase was a better carbon source,and pullulan yield was higher than the treatment with SNase303 glucoamylase after decolor and desalt treatment.The yield of pullulan reached the highest（21.98 g·L-1 and 21.11 g·L-1）when potato syrup produced with β-amylase in DE 50 % was at 80 g·L-1,and CaCO3 at 0.1 g·L-1.The uniform design showed the optimal fermentation condition was CaCO3 0.06 g·L-1,β-amylase in DE 50 % 82.25 g·L-1,26.62 % liquid volume in flask,11.99 % inoculum and pH 7.5.The yield of pullulan reached 28.90 g·L-1 under this condition.

关 键 词：出芽短梗霉 马铃薯水解糖 茁霉多糖 发酵工艺 

分 类 号：TS201.3[轻工技术与工程—食品科学]