固定化细胞发酵—膜蒸馏耦合生产乙醇的研究  

A study on ethanol fermentation by immobilized yeast coupled with membrane distillation

作　　者：姚鹏[1] 马润宇[1] 

机构地区：[1]北京化工大学化学工程学院

出　　处：《北京化工大学学报（自然科学版）》1998年第4期6-10,共5页Journal of Beijing University of Chemical Technology(Natural Science Edition)

摘　　要：对固定化酿酒酵母细胞发酵与膜蒸馏耦合生产乙醇进行了实验研究。首先实验测定了平板膜蒸馏器的分离性能以及游离酵母细胞发酵动力学和固定化酵母细胞发酵动力学；然后将游离细胞发酵罐及固定化细胞反应器分别与平板膜蒸馏装置相耦合构成膜生物反应器，对固定化细胞发酵—膜蒸馏分离乙醇耦合过程进行了研究。在游离酵母细胞发酵中，间歇培养１３ｈ，发酵液中乙醇质量浓度、酵母细胞密度（单位体积发酵液内细胞个数）和葡萄糖转化率、乙醇产率分别为３２ｇ／Ｌ、５７×１０１１个／Ｌ、７７％和３５ｇ／（Ｌ·ｈ）；而在同样培养条件下，用细胞密度为１０１２个／Ｌ凝胶的固定化酵母进行间歇发酵，１２ｈ后的乙醇质量浓度、葡萄糖转化率和乙醇产率分别为３９ｇ／Ｌ、７９％和２２６ｇ／（Ｌ·ｈ）。将固定化酵母细胞发酵与膜蒸馏耦合，乙醇产率可达３００ｇ／（Ｌ·ｈ）。A technique of ethanol fermentation by immobilized saccharomyces cerevisiae coupled with membrane distillation (MD) was studied. A free cell fermentation reactor and an immobilized cell reactor are coupled with a plat gas gap MD module separately. Processes of fermentation of free cell and immobilized cell and coupled process were experimentally evaluated respectively. In the same cultural condition, ethanol productivity of free cell was 3.5g/(L·h) while that of immobilized cell was 22.6g/(L·h). Coupled with MD, ethanol productivity by immobilized yeast was 30.0g/(L·h). With the fermentation proceeding, the permeate flux decreased but the selectivity kept unaltered. The deterioration was likely due to membrane fouling caused by culture medium.

关 键 词：乙醇发酵 固定化细胞 膜蒸馏 反应 分解 耦合 

分 类 号：TS262.2[轻工技术与工程—发酵工程]