胡萝卜表面产纤维素酶细菌初步鉴定分析  

Preliminary Identification and Analysis of Cellulase-producing Strains from Carrot

作　　者：侯进慧[1] 刘全德[1] 高明侠[1] 郭翔[1] 叶骏[1] 

机构地区：[1]徐州工程学院食品(生物)工程学院,江苏徐州221008

出　　处：《食品科学》2010年第5期233-236,共4页Food Science

基　　金：徐州工程学院引进人才科研启动项目;徐州工程学院科研项目(XKY2008110)

摘　　要：从胡萝卜表面筛选到一株产纤维素酶活性较高的菌株HP1。使用PCR方法获得此菌株长度为1435bp的16SrDNA序列。利用生物信息学方法在美国国立生物技术信息中心NCBI基因库比对分析了该序列,发现其与Bacilluscereus相似性最高,为99%。结合菌落观察和生理生化分析,初步确定菌株HP1为Bacillussp.。序列和生化分析显示其是一株新型的芽孢杆菌,其在NCBI上的登录号是:FJ908092。In this study, a strain, HP1, with high cellulase activity was isolated from the surface of carrots. The 16S rDNA fragment of HP1 was identified to be 1435bp by PCR. The sequence was analyzed by bioinformatics method and aligned with sequences published in NCBI Genbank. This strain exhibited 99% similarity with Bacillus cereus. According to colony observation combined with physiological-biochemical analysis, HP1 was preliminarily identified to belong to Bacillus sp., which was a new strain of Bacillus based on sequence and biochemical analyses. The accession number of this strain in NCBI was FJ908092.

关 键 词：胡萝卜 纤维素酶 BACILLUS 16SrDNA 

分 类 号：TS201.3[轻工技术与工程—食品科学]