酶解结合超声波辅助技术提取纯化菊米总黄酮  

Enzymatic Hydrolysis Followed by Ultrasonic-assisted Extraction and Macroporous Resin Adsorption for Purification of Total Flavonoids from Flos Chrysanthemi Indici

作　　者：姚超[1] 于海宁[2] 阮晖[1] 何国庆[1] 沈生荣[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310029 [2]浙江工业大学药学院,浙江杭州310014

出　　处：《食品科学》2010年第6期48-51,共4页Food Science

基　　金：浙江省科技厅重大招标项目(2008C02005-4)

摘　　要：采用纤维素酶酶解结合超声波辅助提取,通过大孔树脂分离、纯化菊米中的黄酮类化合物,并通过单因素试验和正交试验进行工艺优化。结果表明:纤维素酶最佳酶解条件为料液比1:10(g/mL)、酶用量1.5%、酶解温度40℃、pH6.0、酶解时间6h;超声波辅助最佳提取条件为乙醇体积分数70%、提取时间0.5h。以自然流速过大孔树脂D-101柱,经水洗除杂、60%乙醇溶液洗脱,获得菊米提取物得率达7%,黄酮类物质含量50%以上。Cellulase hydrolysis followed by ultrasonic-assisted extraction was adopted to extract crude total flavonoids from Flos Chrysanthemi Indici.The optimal cellulase hydrolysis and ultrasonic-assisted extraction processes were both investigated by orthogonal array design.Besides,the purification of the crude total flavonoids with D-101 macroporous resin column was explored.Results showed that the optimal cellulase hydrolysis could be achieved via hydrolysis for 6 h at 40 ℃,pH 6.0,1：10 material/liquid ratio（g/mL） and 1.5% enzyme dose,and the use of 70% ethanol solution to extract the cellulase hydrolysate of Flos Chrysanthemi Indici for 0.5 h resulted in an optimal extraction of total flavonoids.The purification process consisting of adsorption of total flavonoids onto D-101 macroporous resin column at natural flow rate,washing of the column with water for the removal of impurities and desorption with 60% ethanol gave a recovery and purity of total flavonoids of 7% and more than 50%,respectively.

关 键 词：黄酮类物质 超声波 纤维素酶 大孔树脂 菊米 

分 类 号：R248.2[医药卫生—中医临床基础]