戊糖乳杆菌WH12-2-1产细菌素条件的优化  

Optimation of condition of bacteriocin production by Lactobacillus pentosus WH12-2-1

作　　者：苏芳[1] 李莉[1] 罗斌[1] 孟和毕力格[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院、乳品生物技术与工程教育部重点实验室,呼和浩特010018

出　　处：《中国乳品工业》2010年第3期10-15,共6页China Dairy Industry

摘　　要：以分离自内蒙古传统乳制品中的戊糖乳杆菌(Lactobacillus pentosus)WH12-2-1为试验菌株,研究其产细菌素的最佳条件,以提高该菌产细菌素的能力。对碳源、氮源等培养基成分及培养条件进行优化,并采用响应平面法确定其最佳的碳源、氮源和缓冲盐的质量浓度。结果表明,L.pentosus WH12-2-1在37℃(pH值为7.0),抑菌活性最佳,培养基成分为:大豆蛋白胨10g/L,牛肉膏5g/L,酵母粉5g/L,葡萄糖30g/L,柠檬酸钠6g/L,磷酸氢二钾6g/L,乙酸钠15g/L,Tween-802g/L,硫酸镁1g/L,硫酸锰50mg/L,硫酸亚铁50mg/L。此条件下,细菌素的产量是原来的3.38倍。As one strain was isolated from traditional fermented dairy product of Inner Mongolia, Lactobacillus pentosus WH12-2-1 was investigated for its optimal condition of bacteriocin production. Medium components such as carbon and nitrogen sources and cultivation condition were optimized, the optimal concentration of buffer salt, carbon and nitrogen sources were determined by using response surface method. The results showed that Lactobacillus pentosus WH12-2-1 optimal culture medium components was consist of 10 g/L soy peptone, 5 g/L beef extract, 5 g/L yeast extract, 30 g/L glucose, 6 g/L sodium citrate, 6 g/L K2HPO4, 15 g/L CH3COONa, 2 g/L Tween-80, 1 g/L MgSO4, 50 mg/L MnSO4 and 50mg/L FeSO4 at 37℃ with start pH 7.0.Under optimized condition, Lactobacillus pentosus WH12-2-1 produced 3.38-fold higher amount ofbacteriocin than the un-optimized condition.

关 键 词：糖乳杆菌WH12—2—1 细菌素 优化 

分 类 号：Q93-335[生物学—微生物学]