宇佐美曲霉酸性蛋白酶发酵工艺研究  

STUDIES ON THE ACID PROTEASE FERMENTATION TECHNIQUE OF USAMII L336

作　　者：李永泉[1] 贺筱蓉[1] 翁醒华[1] 胡华萃 林满喜 潘波 

机构地区：[1]浙江大学(西溪校区)生命科学学院

出　　处：《化学反应工程与工艺》1998年第4期382-387,共6页Chemical Reaction Engineering and Technology

摘　　要：宇佐美曲霉Ｌ３３５是一株分泌高单位酸性蛋白酶的生产菌，本研究采用单因素搜索和正交试验，通过摇瓶发酵和２Ｌ搅拌罐发酵，对其发酵工艺进行优化。结果表明，较适宜的发酵培养基（ｇ／１００ｍＬ）为黄豆粉５．０、玉米粉１．２、鱼粉０．８、ＣａＣｌ２０．５、Ｎａ２ＨＰＯ４０．２、ＮＨ４Ｃｌ１．０、蚕蛹水解液１０．０；通气量控制２５ｈ前为１∶０．４ｖ／ｖ／ｍ、２５－５０ｈ１∶１．０ｖ／ｖ／ｍ、５０ｈ后１∶１．３ｖ／ｖ／ｍ；孢子接种，３１±１℃培养８８ｈ。在上述较优发酵条件下，酸性蛋白酶活力在６１００ｕ／ｍＬ以上，发酵单位居国内领先地位。Asp. usamii L336 strain could produce high yield of acid protease. For optimizing its fermentation conditions, a single factor searching and orthogonal test were carried out in flask and 2L agitation fermentor. The experiment results showed that ezyme activity of acid protease was over 6100 u/mL under the optimum condition. This fermentation unit is in the lead level in China.

关 键 词：宇佐美曲酶L336 发酵工艺 优化 酸性蛋白酶 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]