凉山地区苦荞酚类物质的提取及抗氧化能力研究  

Phenols Extraction and Antioxidant Capacity of Tartary Buckwheat in Liangshan Region

作　　者：李海萍[1] 王敏[1] 柴岩[2] 王鹏科[2] 王安虎[3] 芦淑娟[1] 臧盛[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]西北农林科技大学农学院,陕西杨凌712100 [3]四川省西昌学院农学系,四川西昌615013

出　　处：《安徽农业科学》2010年第10期5097-5100,共4页Journal of Anhui Agricultural Sciences

基　　金：国家"十一五"科技支撑计划项目(2006BAD02B206)资助

摘　　要：[目的]筛选出凉山地区抗氧化性能最佳的苦荞品种,为苦荞品种选育和开发提供科学依据。[方法]选择凉山地区的19种苦荞为试验材料,将其种子粉碎后过40目筛,得到苦荞粉。提取苦荞粉中的自由酚和结合酚,并测定其中多酚、黄酮和芦丁的含量。[结果]苦荞总酚含量为5810.94～10255.01μmolGAeq/100gDW,自由酚含量为5683.50～10107.82μmolGAeq/100gDW,结合酚含量为56.14～268.27μmolGAeq/100gDW,其中自由酚含量占总酚的98%～99%;总黄酮含量为707.32～1187.58mgRUeq/100gDW;苦荞芦丁含量为638.91～1263.52mg/100gDW,芦丁含量对DPPH.(R2=0.816)和ABTS.+的清除能力(R2=0.763)均呈线性相关。[结论]晋苦2号芦丁含量最高、抗氧化能力最强。[Objective] To screen out varieties of Tartary buckwheat with oxidation resistance in Liangshan region,and provide a scientific basis for the breeding and development.[Method] A total of 19 Buckwheat varieties in Liangshan region were selected as experimental materials.After their seeds flour were respectively milled and passed through a 40 mesh sieve,the free phenols and combined phenols were extracted separately by 80% acetone and ethyl acetate and the content of total phenolics,flavonoids and rutin were then determined.[Result] The results showed that the total phenolic contents,free phenol contents and combined phenol contents were 5 810.94-10 255.01,5 683.5-10 107.82 and 56.14-268.27 μmol Gallic acid equivalents/100 g of whole grain flour,respectively.The contents of free phenol accounted for 98%-99% of total phenolics content.The total flavonoids were ranged from 707.32 to 1 187.58 mg Rutin equivalents/100 g of whole grain flour.The rutin contents were ranged from 638.91-1 263.52 mg /100g DW.Significant and positive linear correlations were found between rutin contents and scavenging ability of DPPH· （R2= 0.816） and ABTS·＋ （R2 =0.763）.[Conclusion] Jinku-2 with maximum content of rutin shows the strongest antioxidant ability.

关 键 词：苦荞 总酚 黄酮 芦丁 抗氧化能力 

分 类 号：R284.2[医药卫生—中药学]