MBTH法测木糖含量的研究  

Study on determination of xylose by MBTH method

作　　者：张永勤[1] 王哲平[1] 徐杰[2] 薛长湖[2] 詹志春 

机构地区：[1]青岛科技大学化工学院,青岛266042 [2]中国海洋大学食品科学与工程学院,青岛266003 [3]武汉新华扬生物股份有限公司,武汉430074

出　　处：《食品科技》2010年第4期247-250,共4页Food Science and Technology

摘　　要：采用3-甲基-2-苯并噻唑酮腙(MBTH)比色法测定木糖含量,确定了测定木糖的最佳条件:600μL MBTH试剂加入到1.2mL木糖溶液(其中含有600μL 0.5mol/L NaOH)中,80℃水浴加热15min,趁热加入硫酸铁铵试剂,冷却后在650nm下测定吸光度A值。该方法测木糖的检测限为0.3μg/mL,检测浓度范围为1.0～29.2μg/mL。Xylose was determined by the method of 3-methyl-2-benzothiazolinone hydrozone（MBTH）.The optimal protocols were：600 μL MBTH reagent was added to 1.2 mL xylose solution（containing 600 μL 0.5 N NaOH）,heated for 15min at 80 ℃ in water bath,while hot,mixed with FeNH4（SO4）2 reagent,measured at 650 nm.The detection limit was 0.3 μg/mL,and the work concentration range was 1.0～29.2 μg/mL.

关 键 词：3-甲基-2-苯并噻唑酮腙(MBTH) 木糖 比色法 波长 

分 类 号：TS207.3[轻工技术与工程—食品科学]