阪崎肠杆菌脉冲场凝胶电泳分型及耐药研究  

Pulsed-field Gel Electrophoresis Types and Drug Resistance of Enterobacter sakazakii

作　　者：许龙岩[1] 袁慕云[1] 刘静宇[1] 赵贵明[2] 邹志飞[1] 阳静[1] 焦红[1] 

机构地区：[1]广东出入境检验检疫局技术中心食品实验室,广东广州510623 [2]中国检验检疫科学研究院食品安全研究所,北京100025

出　　处：《食品科学》2010年第7期205-209,共5页Food Science

基　　金：国家质量监督检验检疫总局科研项目(2007IK180)

摘　　要：目的:研究北京、新疆、广东、辽宁等地和新西兰、美国、印度进口食品中分离的阪崎肠杆菌(ES)分子型别和耐药情况。方法:用限制性内切酶XbaⅠ酶切ES染色体DNA进行脉冲场凝胶电泳(PFGE)实验,用BioNumerics软件对不同地区分离的ES进行比对,分析菌株之间的相似度。用Vitek2进行药敏实验,分析ES耐药情况。结果:38株ES(其中ES12为标准菌株)分成37个PFGE型,相似度在25%～100%,未表现出优势带型和地区特异性,而部分食品被遗传紧密相关的ES克隆株污染。药敏实验结果显示37株ES共有9种耐药谱,部分菌株对呋喃类、头孢类、β-内酰胺类耐药。结论:建立的PFGE方法可应用于ES的分子分型和溯源。Objectives：To investigate molecular types and drug resistance patterns of Enterobacter sakazakii isolated from foods from Beijing,Xinjiang,Guangdong,and Liaoning or imported foods from New Zealand,USA,and India.Methods：E.sakazakii chromosomal DNA was digested by restriction endonuclease Xba I and analyzed by pulsed-field gel electrophoresis（PFGE）.PFGE patterns of E.sakazakii strains from different areas were compared using BioNumberics software to analyze the similarity among strains.Antibiotic susceptibility test was carried out using VITEK2 to analyze drug resistance patterns.Results：Totally 38 E.sakazakii strains revealed 37 PFGE patterns with similarity ranged from 25% to 100%.Neither significantly predominant PFGE patterns nor area specificity were observed.Moreover,some food samples were contaminated by genetically related E.sakazakii clonal strains.Furthermore,the antibiotic susceptibility test also exhibited 9 drug resistance patterns including resistance to furans,cephalosporins,and β-lactam antibiotics.Conclusion：The PFGE method described above may be used for molecular typing and source tracing of E.sakazakii.

关 键 词：阪崎肠杆菌 脉冲场凝胶电泳 分子分型 食品 耐药 

分 类 号：Q939[生物学—微生物学]