谷氨酸棒杆菌苏氨酸脱氨酶基因敲除及对L-亮氨酸发酵的影响  

Knockout of Threonine Dehydratase Gene in Corynebacterium Glutamicum and Its Influence on L-leucine Fermentation

作　　者：谢希贤[1] 杜军[2] 徐庆阳[1] 刘淑云[1] 陈宁[1] 石维忱[2] 

机构地区：[1]天津科技大学生物工程学院,工业微生物教育部重点实验室,天津300457 [2]中国发酵工业协会,北京100037

出　　处：《中国食品学报》2010年第2期40-45,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家863计划项目(2007AA02Z200);天津市应用基础及前沿技术研究计划(08JCZDJC15400)

摘　　要：L-异亮氨酸是L-亮氨酸发酵的主要副产物,L-异亮氨酸的积累对L-亮氨酸的发酵及提取均产生不利影响。采用基因重组技术敲除L-亮氨酸产生菌-谷氨酸棒杆菌(Corynebacterium glutamicum)TGL8207的苏氨酸脱氨酶基因ilvA,构建了ilvA缺失株谷氨酸棒杆菌(C.glutamicum)TGL8207 ilvA。发酵结果显示:ilvA基因缺失对TGL8207 ilvA的生长影响很小。与供试菌比较,TGL8207 ilvA的L-亮氨酸产量和糖酸转化率分别提高了8.4%和1.9%。L-isoleucine is the main by-product in L-leucine fermentation.The accumulation of L-isoleucine has a negative impact on L-leucine fermentation and retrieval.In this study,threonine deaminase gene ilvA of the L-leucine producing train C.glutamicum TGL8207 was knocked out using gene recombination technology and a ilvA deletion mutant C.glutamicum TGL8207 ilvA was constructed.The fermentation result indicated that ilvA gene deletion has little effect on the growth of TGL8207 ilvA.Compared with the original strain,the L-leucine yield and sugar/acid conversion rate of TGL8207 ilvA were increased by 8.4% and 1.9%,respectively.

关 键 词：谷氨酸棒杆菌 L-亮氨酸 苏氨酸脱氨酶 基因敲除 发酵 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]