乳制品中纳他霉素的液相色谱检测方法  

Dairy Products Natamycin Liquid Chromatography Detection Methods

作　　者：闫磊[1] 刘宁[1] 孙亚范[1] 王春天[1] 

机构地区：[1]天津市乳品食品监测中心,天津300381

出　　处：《食品研究与开发》2010年第4期111-112,125,共3页Food Research and Development

摘　　要：建立一种测定乳制品中纳他霉素含量的高效液相色谱法。样品经甲醇萃取后进样,采用C18250mm×4.6mm色谱柱分离,流动相为甲醇:水=6∶4(体积比),紫外检测;A-R303nm,RSD为0.56%～0.82%(n=5),标准曲线R=0.9998,平均回收率分别为97.89%,最低检出限为0.03mg/kg。Determination of the establishment of a dairy Natamycin content in the high-performance liquid chromatography. The sample was less like methanol extraction, using C18 250 mm×4.6 mm separation column, mobile phase was methanol： water=6∶4（volume ratio）,UV detector; A-R303nm, RSD 0.56 %-0. 82 %（n=5）, standard curve,R=0.999 8, average recoveries were 97.89 %, the minimum detection limit of 0.03 mg/kg.

关 键 词：高效液相色谱 纳他霉素 乳制品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]