气相色谱法测定发酵液中胆固醇的含量  

Content Determination of Cholesterol in Fermentation Solution by Gas Chromatography

作　　者：刘长建[1] 姜波[1] 安晓雯[1] 赵轶男[1] 刘秋[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600

出　　处：《微生物学杂志》2009年第6期97-99,共3页Journal of Microbiology

基　　金：国家自然科学基金(30671398);NSFC-KOSEF国际合作项目(30711140389)

摘　　要：气相色谱法测定了发酵液中胆固醇的含量。采用Rtx-1（100%二甲基聚硅氧烷）毛细管色谱柱、FID检测器进行分析测定。当胆固醇浓度在0～200μg/mL范围内与色谱峰面积呈良好线性关系（r=0.9981）。方法的平均回收率为99.38%,相对标准偏差为0.75%（n=8）,最低检出限可达到0.816μg/mL。气相色谱法作为发酵液中胆固醇含量的快速测定方法,具有试剂用量少、操作简单、结果准确等特点。Content of cholesterol in fermentation solution was determined by gas chromatography.FID and Rtx-1（100% dimethyl polysiloxane） capillary column were used to carry out the analysis and tests.When cholesterol concentration was in fine linear relation（r=0.998 1） with chromatogram peak area within the range of 0～200 μg/mL.The average recovery rate of cholesterol was 99.38% with the relative standard deviation as low as 0.75%（n=8）.Determination limit of this method could amount to 0.816 μg/mL.Gas chromatography as a rapid testing method for cholesterol content in fermentation solution possessed traits of small amount of reagent,easy operation and accurate results etc.

关 键 词：气相色谱法 胆固醇 发酵液 

分 类 号：TQ920[轻工技术与工程—发酵工程]