绿衣观音土曲中霉菌糖化酶活力的研究  

Research of Glucoamylase Activity of Molds in Green-Covering Guanyin Taqu

作　　者：李锐利[1] 方尚玲[1] 陈茂彬[1] 刘鸿[1] 

机构地区：[1]发酵工程省部共建教育部重点实验室,湖北省食品发酵工程技术研究中心,湖北武汉430068

出　　处：《酿酒》2010年第1期50-52,共3页Liquor Making

基　　金：湖北省自然科学基金项目资助(2008CDB063)

摘　　要：从绿衣观音土曲中分离得到一株棒曲霉,一株黑曲霉,六株根霉。分别采用平板透明圈、比色法测定所有菌株糖化酶活力。透明圈实验中棒曲霉的菌落直径最小,而透明圈直径最大。比色法测得棒曲霉糖化酶活力为1283.9U/g干曲。两种方法的结果都表明棒曲霉是所分离菌株中糖化酶活力最高的。An Aspergillus clavatus,an Aspergillus niger and six Rhizopus spp.were separated from the Green-covering Guanyin Taqu.Measured the Glucoamylase activity of all strains by the methods of transparent circle and Colorimetric Determination.In the experiment of transparent circle,The Colony diameter of Aspergillus clavatus is the smallest than other molds while the transparent circle is the biggest.Measurement by colorimetry revealed the Aspergillus clavatus has a glucoamylase activity of 1283.9U/g Dry Qu.The results of two methods indicated that Aspergillus clavatus＇ glucoamylase activity was the highest among all the strains.

关 键 词：绿衣观音土曲 棒曲霉 糖化酶活力 比色法 

分 类 号：TQ925[轻工技术与工程—发酵工程] TS207.3