米糠抗氧化肽对D-半乳糖致衰小鼠肝线粒体的保护  

Protective effect of rice bran antioxidant peptides on liver mitochondria in aging mice induced by D-galactose

作　　者：付岩松[1] 罗霞[1] 张心昱[2] 宗立立[3] 樊金娟[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]中国科学院地理科学与资源研究所,北京100101 [3]吉林农业大学农学院,吉林长春130118

出　　处：《食品工业科技》2010年第6期310-312,316,共4页Science and Technology of Food Industry

基　　金：辽宁省教育厅重点实验室项目(2008S207)

摘　　要：目的:观察米糠抗氧化肽对D-半乳糖(D-gal)致衰小鼠肝线粒体的保护作用,并初步探讨其机制。方法:昆明小鼠60只随机分成对照组、衰老模型组、米糠抗氧化肽低、中、高剂量治疗组。衰老模型组每日颈背部皮下注射D-gal100mg/kg·bw;米糠抗氧化肽各组除注射D-gal外,分别灌胃抗氧化肽100、200、500mg/kg·bw;对照组每日皮下注射等量生理盐水,连续6w。利用试剂盒分别检测各组小鼠肝线粒体锰-超氧化物歧化酶(Mn-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)及丙二醛(MDA)含量。结果:与衰老模型组相比,米糠抗氧化肽可明显增强小鼠肝线粒体Mn-SOD、GSH-Px、CAT及T-AOC活性,显著抑制MDA含量的上升(P<0.05或P<0.01),并呈量-效关系。结论:米糠抗氧化肽能够显著提高致衰小鼠的抗氧化能力,减轻肝线粒体损伤,其机制可能与该肽直接清除体内自由基、提高机体抗氧化酶活性以及对抗脂质过氧化反应有关。Objective：To observe the protective effect of rice bran antioxidant peptides on liver mitochondria in aging mice induced by D-galactose（D-gal）, and explore the possible mechanism preliminarily.Method ：Sixty Kunming mice were randomly divided into five groups, namely young control group, aging model group and three rice bran peptides-treated groups.The aging model mice were daily given subcutaneous injection of D-gal on the nape back （100mg/kg · bw）.Besides,mice in three rice bran peptides-treated groups were daily perfuse respectively with the dose of 100,200 and 500mg/kg - bw of rice bran antioxidant peptides for 6 weeks continuously,and those in the young control group were daily given subcutaneous injection of saline of the same volume for 6 weeks continuously.The activities of manganese- superoxide dimutase （ Mn- SOD ）, glutathione peroxidase （ GSH- Px）, catalase（CAT）, total anti- oxidation capacity （T-AOC）, and the content of malonadehide （MDA） in liver mitochondria of all mice were measured by the detection kits.Result：Compared with aging model group, rice bran peptides could markedly enhance the activities of Mn-SOD, GSH-Px, CAT and T-AOC, and significantly inhibit the rise of MDA content（P 〈 0.05 or P 〈 0.0! ）, there was a relationship of dose-effect. Conclusion： Rice bran antioxidant peptides can significantly improve the anti-oxidation function and inhibit liver mitochondria damage in aging mice induced by D-gal.The mechanism is probably mediated with the scavenging activity of the peptides on free radical and the increase of the activities of antioxidative enzymes,so lipid peroxidation is prevented.

关 键 词：米糠 抗氧化肽 肝线粒体 衰老模型 

分 类 号：TS201.4[轻工技术与工程—食品科学]