大肠杆菌表达包涵体蛋白体外复性研究进展  

Development of in-vitro refolding of inclusion body proteins in E.coli

作　　者：赵喜红[1,2] 何小维[1,2] 李文美[1,2] 杨连生[1] 王继华[1,2] 彭运平[1,2] 刘晓云[1,2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]广州万孚生物技术有限公司,广东广州510640

出　　处：《食品工业科技》2010年第6期379-383,共5页Science and Technology of Food Industry

基　　金：广东省部产学研结合项目(2006D90504011);广东省科技攻关项目(2006B40101013);国家科技部火炬计划项目(2007GH020171)

摘　　要：包涵体形成是大肠杆菌表达重组蛋白常见的问题。因此,为获得大量的活性蛋白而对其包涵体进行复性的研究引起了人们极大兴趣。本文综述了近年来发展起来的低分子量添加物、反胶团、分子伴侣、层析复性等新的复性方法、复性检测方法和体外复性机制。Inclusion body production is a common theme in recombinant protein technology in E. coll. Hence, refolding of these inclusion body proteins is a field of increasing interest for gaining large amounts of active proteins.Recent developments of refolding procedures including the inhibition of aggregation during refolding by the application of low molecular weight additives, reverse micelles, artificial chaperone, chromatographic refolding techniques,determination methods and the mechanism of protein refolding in vitro were reviewed in this paper.

关 键 词：包涵体 蛋白复性 重组蛋白 

分 类 号：TS201.3[轻工技术与工程—食品科学]