假丝酵母99-125脂肪酶喷雾干燥工艺及催化性质的研究  

Spray drying process for formulation of lipase from Candida sp. 99-125 and catalytic properties the powdered lipase

作　　者：潘雪[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院北京市生物加工过程重点实验室,北京100029

出　　处：《生物加工过程》2010年第2期30-35,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20636010;20876011);北京市科技计划资助项目(D0205004040211);国家重点基础研究发展计划(973计划)资助项目(2007CB714305;2007CB714304);国家高技术研究发展计划(863计划)资助项目(2006AA020203);北京市自然科学基金资助(2071002);北京市教育委员会共建项目专项资助

摘　　要：利用响应面法对假丝酵母脂肪酶喷雾干燥工艺条件进行优化,考察进口温度、雾化速度、保护剂含量对脂肪酶活力收率的影响。确定了最佳喷雾条件:保护剂为10～15 g/L的阿拉伯胶,进口温度115～120℃,雾化速度0.4 L/h,可得到收率最高为60.5%的脂肪酶酶粉。制得的固定化酶用于手性拆分(R,S)-1-苯乙醇,光学产率最高可达到53.6%;用于催化合成生物柴油,转化率最高可达到90.2%。在4、30℃下密封保存,半衰期可分别达到15个月、3个月。The process condition for spray drying of Candida sp.99～125 lipase was optimized by a response surface methodology.The effects of the entrance temperature,the atomization speed,and additives were investigated.The optimized process parameters were as follows： the addition of the arabic gum was 1.0～1.5%,the entrance temperature was 115～120 ℃,and the atomization speed was 0.4 L/h,the highest yield of the enzyme activity could achieve 60.5%.The immobilized lipase was used for the resolution of rac-2-phenylethanol,the reaction obtained an e.e.53.6%;for the transesterification of oil with methanol,the conversion rate reached 90.2%.The half-life of spray-dried lipase powder under 4 and 30 ℃ were 15 and 3 months,respectively.

关 键 词：假丝酵母 脂肪酶 响应面法 喷雾干燥 

分 类 号：Q556.1[生物学—生物化学] TQ922.1[轻工技术与工程—发酵工程]