一株细菌ps-5胞外多糖抗氧化性能研究  

Study on antioxidation effect of the exopolysaccharide from a bacteria ps-5

作　　者：刘莎[1] 叶淑红[1] 王际辉[1] 曹方[1] 陈富远 

机构地区：[1]大连工业大学生物与食品工程学院,大连116034 [2]大连础明集团有限公司,大连116034

出　　处：《食品科技》2010年第6期111-114,共4页Food Science and Technology

基　　金：辽宁省教育厅科学技术研究项目(20060178);辽宁省创新团队计划(2007T009);辽宁农业产业化计划(2007211001;2008301029);大连市科技计划(2008B12NC059)

摘　　要：土壤细菌ps-5产胞外多糖经乙醇沉淀、Sevage法脱蛋白、透析、冷冻干燥等方法分离和纯化后得到粗多糖,多糖含量为0.3 mg/mL。该多糖对.OH和O2-.有较强的清除能力,当清除率达50%时所需浓度分别为0.0476、0.2770 mg/mL,而在同等条件下,使用Vc清除率达到50%所需浓度分别为0.0440、5.70 mg/mL。研究多糖对油脂的抗氧化活性,结果表明,ps-5胞外多糖可有效抑制动植物油脂的氧化。An exopolysaccharide（EPS） was extracted from a bacteria ps-5 by ethanol precipitation and pu-rified by Sevage method,dialysis and freeze drying.The crude product contained 0.3 mg/mL of polysaccharide.The polysaccharide had the scavenging action on.OH and O2-..The scavenging activity to superoxide anion and hydroxyl free radicals was represented by the content scavenged 50% of all radicals（IC50）.The IC50 of exopolysaccharide to superoxide anion and hydroxyl free radicals were 0.0476 mg/mL and 0.2770 mg/mL res-pectively.The IC50 of Vc were 0.0440 mg/mL and 5.70 mg/mL under the same condition.At the same time,this exopolysaccharide had the activity of oxidation resistance to animal fat and vegetable oil effectively.

关 键 词：细菌 胞外多糖 抗氧化 

分 类 号：TS202.3[轻工技术与工程—食品科学]