醋酸杆菌α-乙酰乳酸脱羧酶基因克隆、表达及在啤酒发酵中的作用  

Cloning and Expression of α Acetolactate Decarboxylase Gene form Acetobacter aceti and Maturation of Beer with the Enzyme

作　　者：尹东[1] 卢大宁[1] 曾庆华[1] 黄百渠[1] 李彦舫[2] 

机构地区：[1]东北师范大学遗传与细胞研究所 [2]中国人民解放军农牧大学

出　　处：《中国生物化学与分子生物学报》1999年第1期24-26,共3页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：根据α－乙酰乳酸脱羧酶（α－ａｃｅｔｏｌａｃｔａｔｅｄｅｃａｒｂｏｘｙｌａｓｅ，α－ＡＬＤＣ）基因的碱基序列，用ＰＣＲ方法从醋酸杆菌（Ａｃｅｔｏｂａｃｔｅｒａｃｅｔｉ）中克隆出了０．９９ｋｂ的ＤＮＡ片段，经ＤＮＡ测序后证明该片段是α－乙酰乳酸脱羧酶基因．将该基因重组到质粒ｐＢＶ２２０中，转化大肠杆菌，筛选获得具有α－ＡＬＤＣ活性的重组子菌株．酶活检测表明，重组子细胞表达的α－ＡＬＤＣ活性是供体菌的１１００倍．将得到的α－ＡＬＤＣ粗提后用于啤酒发酵试验。Alpha acetolactate decarboxylase (α ALDC) gene was isolated from Acetobacter aceti by PCR amplification.The 0 99 kb amplified fragment was confirmed to be the α ALDC gene by DNA sequencing.The gene was inserted into the vector pBV220 to construct an expression plasmid pBVYⅢ and the recombinant plasmid expressed α ALDC in E.coli DH5α.The α ALDC activity of the recombinant bacterium was 1100 fold higher than that of the host Acetobacter aceti .The α ALDC was shown to be able to reduce diacetyl during beer fermentation.

关 键 词：醋酸杆菌 α-ALDC 基因克隆 啤酒发酵 

分 类 号：TS261.1[轻工技术与工程—发酵工程]