乳清蛋白肽对衰老小鼠抗氧化能力的影响  

EFFECT OF WHEY PROTEIN PEPTIDES ON ANTIOXIDANT ABILITIES IN AGED MICE

作　　者：包怡红[1] 李锐达[1] 梁雪[1] 秦蕾[1] 李文星[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《营养学报》2010年第3期239-241,共3页Acta Nutrimenta Sinica

基　　金：黑龙江省博士后科研启动资金(No.LBH-Q08142)

摘　　要：目的观察乳清蛋白肽(whey protein peptides,WPP)对衰老小鼠体内抗氧化能力的影响。方法 D-半乳糖连续颈部注射制作亚急性衰老小鼠模型。以VE为阳性对照,灌胃给予WPP100、200、400mg/(kgbw·d),45d后观察其对小鼠血清、肝脏、脑组织中过氧化氢酶(CAT)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量的影响。结果衰老小鼠血浆、肝脏、脑组织匀浆中CAT、SOD、GSH-Px含量均显著降低,MDA含量显著升高,与正常小鼠比较差异显著;200、400mg/(kgbw·d)WPP可以提高衰老小鼠血浆、肝脏、脑组织中CAT、SOD、GSH-Px含量,降低MDA含量,与衰老模型组比较差异有显著性。结论 WPP能显著提高亚急性衰老小鼠的抗氧化能力,具有量效关系。Objective To observe the antioxidant activities of whey protein peptides （WPP） in aged mice.Method The subacute aged model mice were made by neck back subcutaneous injection of D-galactose every day.Compared with VE as positive control,the mice were given three different doses of WPP,100,200,400 mg/（kg bw·d） respectively,the effect of WPP on the content of catalase （CAT）,malondialdehyde （MDA）,superoxide dismutase （SOD）,glutathione peroxidase （GSH-PX）in serum,liver and brain were observed after 45 d.Results The CAT,SOD and GSH-PX activities in aged model group were significantly decreased,but MDA was significantly increased as compared to normal mice.While in the aged mice treated with WPP 200 and 400 mg/（kg bw·d）,the activity of CAT,SOD and GSH-PX were significantly increased and the content of MDA significantly decreased as compared to aged mice.Conclusion WPP shows dose-dependant antioxidant effect in aged mice.

关 键 词：乳清蛋白肽 抗氧化 过氧化氢酶 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶 

分 类 号：R151[医药卫生—营养与食品卫生学]