高压CO_2酸化杀菌机理的研究  

Sterilization Mechanism of High-pressure Carbon Dioxide-caused Acidification

作　　者：周先汉[1] 宋俊骅[1] 曾庆梅[2] 张安[1] 程丽梅[1] 胡行军[1] 邹旭鹏[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]合肥工业大学农产品生物化工教育部工程研究中心,安徽合肥230009

出　　处：《食品科学》2010年第11期11-14,共4页Food Science

基　　金：国家自然科学基金项目(30871739;30571304);安徽省教育厅重点科研项目(KJ2007A099)

摘　　要：以大肠杆菌及其低活性P-AT Pase型和F-AT Pase型大肠杆菌为研究对象,通过对比实验初步研究高压CO2杀菌过程的酸化机理。结果表明,高压CO2对突变型大肠杆菌的致死效果比对正常型大肠杆菌的致死效果都要好,H+-AT Pase的活性对杀菌效果具有显著性影响,说明在高压CO2杀菌过程中,由于CO2气体渗透入细菌的细胞内,使胞内发生酸化,致使细菌死亡。The sterilization mechanism of high-pressure carbon dioxide-caused acidification was studied using a wild-type strain of Escherichia coli and two mutant strains derived from it with low activity of P-ATPase or F-ATPase.The results showed that high-pressure carbon dioxide under the identical condition exhibited better lethal effect on both the mutant strains than on the wild-type strain.Meanwhile,a remarkable effect of H＋-ATPase activity on sterilization was observed.Therefore,these investigations indicated that high-pressure carbon dioxide could result in intercellular acidification to cause bacterial death during sterilization.

关 键 词：高压CO2 杀菌机理 P-AT Pase F-AT Pase 大肠杆菌 

分 类 号：TS205.9[轻工技术与工程—食品科学]