生物吸附剂耐盐性鲁氏酵母培养基的优化  

Optimization of medium for biosorbent salt-torlerance Zygosaccharomyces rouxii

作　　者：姜维[1] 徐莹[1] 刘文磊[1] 汪东风[1] 栾红蕾[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国酿造》2010年第7期32-36,共5页China Brewing

基　　金："十一五"国家科技支撑计划重大项目(2008BAD94B09);教育部博士点基金新教师项目(200804231032);教育部博士点基金项目(20070423020)

摘　　要：耐盐性鲁氏酵母可以用作吸附水中重金属的生物吸附剂。为了获得大量的耐盐性鲁氏酵母,采用响应面分析法对鲁氏酵母CICC1379培养基进行了优化。单因素试验结果表明,最佳碳源为葡萄糖和麦芽糖、最佳氮源为酵母膏。部分因子试验结果表明,葡萄糖、麦芽糖和酵母膏对生物量影响显著,硫酸镁、氯化钠、磷酸二氢钾和氯化钙对生物量影响不显著。由中心组合试验及响应面分析优化确定培养基组成:葡萄糖5.0%,麦芽糖3.3%,酵母膏2.8%,硫酸镁0.3%和氯化钠0.6%,得到二次拟合回归模型,并预测最大生物量为10.13 mg/mL。验证试验表明了该模型的正确性。利用该优化培养基培养得到的生物量比麦芽汁、YEPD、YM培养基分别增加了44.0%、82.8%和102.6%,提升了鲁氏酵母CICC1379作为生物吸附剂的应用潜力。Biomass of salt-tolerance Zygosaceharomyces rouxii can be used as biosorbent of heavy metal ions in polluted water. In order to obtain more biomass, medium for Z. rouxii CICC 1379 was optimized with central composite design and response surface analysis method. Results of single factor experiments indicated that the best carbon sources were glucose and maltose and the best nitrogen source was yeast extract. The results of two-factor factorial design indicated that contents of glucose, maltose, and yeast extract significantly affected yield of biomass, and content of MgSO4, NaCI, KH2PO4 and CaC12 had no significant effect. The optimum medium was obtained with central composite design and response surface analysis method as follows： glucose 5.0%, maltose 3.3%, yeast extract 2.8%, MgSO4 0.3% and NaCl 0.6%. The maximum yield of biomass was predicted to be 10.13 mg/mL with model of quadratic regression. It was validated by the yeast biomass with 10.1 lmg/mL from the optimized medium in shaking flasks, which verified the effectiveness of the model. Yield of biomass with the optimum medium was increased by 44.0%, 82.8% and 102.6% to wort medium, YEPD and YM, respectively. This optimization medium increased potentiality of applications of Z. rouxii CICC 1379 as the biosorbent.

关 键 词：耐盐性鲁氏酵母 培养基:响应面 优化 

分 类 号：Q93-335[生物学—微生物学]