食品中大肠杆菌O157的PCR检测与wzy基因的测序分析  

PCR Detection of Escherichia coli O157 in Food and Sequencing of wzy Gene

作　　者：李睿[1] 张忠美[1] 戴诗皎[1] 孙言凤[1] 黄万琪[1] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023

出　　处：《食品科学》2010年第12期193-196,共4页Food Science

基　　金：湖北省自然科学基金重点项目(2009CDA118)

摘　　要：大肠杆菌O157:H7和O157:H-是重要食品致病菌。从食品中分离得到一株O157:H7菌株EC5。将该菌株PCR扩增wzy基因全长并克隆测序。结果表明,wzy基因序列与大肠杆菌O157:H7标准株EDL933有100%同源性。从NCBIGenBank数据库下载O157菌株wzy基因序列,针对保守序列设计引物并扩增O157菌株和非O157菌株。PCR结果表明,wzy基因特异性强,可作为检测O157菌株的标志基因。本研究为开发大肠杆菌O157的分子检测技术提供新的研究思路。A strain of Escherichia coli O157：H7 was isolated from fresh beef and named EC5.EC5 was subjected to PCR amplification and sequencing of full-length wzy gene.There was a 100% similarity between the wzy gene sequence of EC5 and that of Escherichia coli O157：H7 EDL933.Specific primers were designed based on the conserved locus of wzy gene sequences known from NCBI GenBank database for differentiating O157 strains from non-O157 ones by PCR assay.Our findings demonstrate that the wzy gene amplified by PCR is highly specific and provides a promising marker gene for the detection of Escherichia coli O157：H7.

关 键 词：大肠杆菌O157 PCR检测 wzy基因 测序 

分 类 号：R155.5[医药卫生—营养与食品卫生学]