采用HPLC-DAD-ESIMS技术鉴定紫山药中的花色苷成分  

Identification of Anthocyanins in Purple Yam by HPLC-DAD-ESIMS

作　　者：于东[1] 叶兴乾[1] 方忠祥[1] 陈桂星[2] 许荷法 

机构地区：[1]浙江大学食品科学与营养系,杭州310029 [2]台州市黄岩区农机总站,浙江台州318020

出　　处：《中国食品学报》2010年第3期213-218,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省重大科技专项(No.2008C02005-2);浙江省科技计划面上重点项目(No.2008C22013)

摘　　要：采用大孔树酯对浙江台州黄岩紫山药中的花色苷进行分离,并用高压液相色谱-二极管阵列检测-质谱检测技术对其中的主要花色苷进行结构鉴定。通过差异pH法分析紫山药中花色苷的含量为28.8mg/kg。紫山药中4种主要的花色苷可能是:矢车菊素-3-葡萄糖苷或半乳糖苷的双咖啡酸酰化物,矢车菊素-3-二糖苷的芥子酸酰化物,矢车菊素-3-二糖苷阿魏酸的酰化物,芍药色素-3-二糖苷的芥子酸酰化物。Anthocyanins extracted from purple yam （from Huangyan district,Taizhou city,Zhejiang province） were isolated by macroporous resin and the main 4 types of them were tentatively identified by the method of HPLC-DADESIMS.The total content of anthocyanins is 28.8 mg/kg in the yam,using pH differential method.The 4 main anthocyanins may be cyanidin-3-glucoside （galactoside） acylated with two caffeic acids,cyanidin-3-diglucoside acylated with sinapic acid,cyanidin-3-diglucoside acylated with ferlulic acid and peonidin-3-diglucoside acylated with sinapic acid.

关 键 词：紫山药 花色苷 液相色谱-二级管阵列检测-质谱联用 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]