响应面法优化木聚糖酶发酵培养基的研究  

Optimization of xylanase fermentation by response surface methodology

作　　者：武振军[1] 石楠[1] 吴启赐[1] 单咸旸[1] 赵宗松[1] 万红贵[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《中国酿造》2010年第8期77-80,共4页China Brewing

摘　　要：采用响应面法对毕赤酵母发酵产木聚糖酶的培养基进行了优化。首先利用Plackett Burman试验设计筛选出影响产酶的3个主要因素,即麸皮水解液浓度、酵母水解液浓度和甲醇添加量。在此基础上用最陡爬坡路径逼近最大响应区域,再利用Box-Behnken试验设计及响应面分析法确定最佳条件。结果表明,麸皮水解液402.5g/L、酵母水解液49.9 g/L和甲醇添加量为28.1mL/L时,木聚糖酶最大理论酶活为6566.79U/mL。经3次试验验证,实际平均酶活与预测酶活相近,比优化前木聚糖酶酶活提高了23.7%。Response surface methodology was used to optimize the fermentation conditions of xylanase by Pichia pastoris. Firstly, the important factors influencing xylanase production were identified by the method of Plackett-Burman as bran hydrolysate, yeast hydrolysate and methanol. The path of steepest ascent was applied to approach the optimal region of the three significant factors. Box-Behnken design and response surface analysis were used to further investigate the optimal central levels and relationships of these factors. By using the quadratic regression model equation and analyz- ing the response surface of xylanase production, the optimal fermentation conditions were determined as followed： bran hydrolysate 402.5 g/L, methanol 28.1 ml/L and yeast hydrolysate 49.9 g/L. Under these conditons, the maximum theoretic activity of xylanase was 6566.79 U/ml. After three verifications, the maximum theoretic value was consistent with mean value of verification test and the xylanase production was increased by 23.7% compare to that before optimization.

关 键 词：响应面分析 发酵优化 木聚糖酶 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]