高效液相色谱-蒸发光散射法测定酶法合成低聚乳果糖的含量  

High-performance Liquid Chromatography with Evaporative Light Scattering Detection for the Determination of Enzymatically Synthesized Lactosucrose

作　　者：代志凯[1,2] 廖春龙[1,2] 印遇龙[1,2,3] 阮征[1,2] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学生命科学与食品工程学院,江西南昌330031 [3]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《食品科学》2010年第16期180-183,共4页Food Science

基　　金：南昌大学"赣江学者奖励计划"项目;中国博士后科学基金资助项目(20080440166);食品科学与技术国家重点实验室探索项目(SKLF-TS-200817);江西省教育厅项目(GJJ09068)

摘　　要：目的:建立简便、准确、灵敏地测定酶法合成中低聚乳果糖含量的液相色谱方法。方法:酶法合成低聚乳果糖的溶液经离心后稀释,使用氨基柱分离,乙腈-水(体积比75:25)为流动相,流速1.0mL/min,蒸发光散射检测器漂移管温度70℃,载气为空气,压力0.35MPa。结果:低聚乳果糖的线性范围为0.1281～4.1000mg/mL(R2=0.9994),最低检出限为0.01mg/mL,平均加标回收率为100.28%,RSD为3.07%。结论:该方法操作简便、快速、准确,用于酶法合成低聚乳果糖的测定,结果令人满意。Objective： To develop a simple, accurate and sensitive high-performance liquid chromatographic （HPLC） method with evaporative light scattering detection （ELSD） for the determination of enzymatically prepared lactosucrose （LS） in our laboratory. Methods： The reaction product containing LS was centrifugated and the supernatant was diluted and separated on a Kromasil NH2 column using acetonitrile/water （75：25, V/V） mixture as mobile phase. The ELSD drift tube temperature was set at 70 ℃ and the air pressure at 0.35 MPa. Results： The linear range of LS was between 0.1281 mg/mL and 4.1000 mg/mL （R2 = 0.9994）. The method detection limit was 0.01 mg/mL. The average spike recovery at 5 levels was 100.28%, with a relative standard deviation （RSD） of 3.07%. Conclusion： This method is simple, fast and accurate, and can be applied to the determination of LS.

关 键 词：高效液相色谱 蒸发光散射检测器 低聚乳果糖 

分 类 号：TS247[轻工技术与工程—制糖工程]