同时蒸馏萃取与固相微萃取法提取馒头中挥发性物质  

Simultaneous Distillation-extraction and Headspace Solid Phase Microextraction Methods for Analysis of Volatiles in Mantou

作　　者：胡丽花[1] 苏东民[1] 苏东海[2] 辛秀兰[2] 

机构地区：[1]河南工业大学粮油食品学院,郑州450052 [2]北京电子科技职业学院生物技术系,北京100029

出　　处：《中国农学通报》2010年第17期98-102,共5页Chinese Agricultural Science Bulletin

基　　金：2009年度北京市自然科学基金资助项目"馒头多菌种发酵机理以及对馒头风味影响的研究"(5093026);河南省重点科技攻关项目"中国传统蒸制面食标准化;连锁化;产业化研究"(0523011000);北京市教委科技计划面上项目"馒头多菌种固态发酵机理以及对馒头品质影响的研究"(KM200900002003)

摘　　要：为分析2种方法萃取的馒头样品中的挥发性物质的差异,采用同时蒸馏萃取法(SDE)和固相微萃取法(SPME)分别提取中国主食馒头样品中挥发性风味成分,经气相色谱—质谱联用仪(GC-MS)分离及鉴定。结果显示:从SDE法提取的样品中检测到25种化合物,SPME法提取的样品中鉴定出50种风味组分,2种方法制备的样品中共有70种挥发性化合物主要有醇、醛、酮和酯等。发现虽然SPME法检测到的化合物种类多于SDE法的,但SPME法提取的样品中化合物的浓度较低,且从样品的总离子流色谱图的分离效果来看,SDE法优于SPME法。The volatiles of Mantou were extracted by simultaneous distillation-extraction （SDE） and headspace solid phase microextraction（SPME） respectively, then were separated and identified by gas chromatography-mass spectrometry （GC-MS） , in order to analysis the different of volatiles extracted by two different methods. The result showed that there are 25 compounds were detected from sample extracted by SDE, and 50 compounds were identified by SPME. Total of 70 species of volatile compounds, including alcohols, aldehydes, ketones, esters et al, were detected from samples which were prepared by two methods. It found that although species of compounds detected by SPME are more than SDE, but concentration of compounds in sample extracted by SPME are lower. From the separation efficiency of total-ion current chromatogram, the extract method of SDE is better than SPME.

关 键 词：同时蒸馏萃取 固相微萃取 气相色谱质谱联用仪 馒头 挥发性物质 

分 类 号：TS211.4[轻工技术与工程—粮食、油脂及植物蛋白工程]