黑曲霉DB056发酵α-鼠李糖苷酶和柚苷酶培养基的优化研究  

Optimization of Culture Medium for Producing α-Rhamnosidase and Naringinase by Aspergillus Niger DB056

作　　者：吴升山[1,2] 蔡慧农[1,2] 苏文金[1,2] 倪辉 李利君[1] 肖安风[1] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]厦门市食品生物工程技术研究中心,福建厦门361021

出　　处：《中国食品学报》2010年第4期193-201,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：福建省青年人才创新基金项目(2007F3073);科技人员服务企业项目(SQ2009GJC4000525);福建省科技计划重点项目(No.2009N0044);厦门市科技项目(3502Z20093023)

摘　　要：目的:优化黑曲霉DB056产α-鼠李糖苷酶和柚苷酶的培养基,提高这两种酶的产量。方法:以α-鼠李糖苷酶活力和柚苷酶活力为指标,研究碳源、氮源和乳化剂TritonX-100对α-鼠李糖苷酶活力和柚苷酶活力的影响,优化黑曲霉DB056摇瓶发酵α-鼠李糖苷酶和柚苷酶的培养基。结果:碳氮源的种类与浓度,乳化剂Tri-tonX-100的浓度对黑曲霉DB056产α-鼠李糖苷酶和柚苷酶有重要影响;黑曲霉DB056产酶的优化培养基是:柚皮苷3.5g/L,玉米浆4g/L,MgSO4·7H2O1.0g/L,KH2PO41.0g/L,KCl0.5g/L,无水CaCl20.1g/L,乳化剂TritonX-1001.0%。此时α-鼠李糖苷酶活力和柚苷酶活力分别为994U/mL和276U/mL,分别比发酵初始培养基提高了42.3%和147.8%。结论:通过培养基优化,大幅度提高了黑曲霉DB056液态深层发酵α-鼠李糖苷酶活力和柚苷酶活力。Objective：To optimize the culture medium of Aspergillus niger DB056 for increasing the yields of α-rhamnosidase and naringinase.Methods：The medium with different composition and concentration of carbon source,nitrogen source and emulsifier Triton X-100 were used in shaking culture Aspergillus niger DB056.The activities of α-rhamnosidase and naringinase were detected to evaluate the effect of medium constituents and their concentrations.Results：Both the types and concentrations of carbon source and nitrogen source and the concentration of emulsifier Triton X-100 showed important impact on the activities of α-rhamnosidase and naringinase.The optimum culture medium was consisted of naringin 3.5 g/L,corn steep liquor 4.0 g/L,MgSO4·7H2O 1.0 g/L,KH2PO4 1.0 g/L,KCl 0.5 g/L,CaCl2 0.1 g/L,emulsifier Triton X-100 1%.Using the optimum medium,the activities of α-rhamnosidase and naringinase increased 1.42fold and 2.48-fold,and the enzyme activity of α-rhamnosidase and naringinase reached to 994 U/mL and 276 U/mL,respectively.Conclusion：After the optimization of medium,the activity of α-rhamnosidase and naringinase were increased significently in liquid deep layer fermentation using Aspergillus niger DB056.

关 键 词：黑曲霉 发酵培养基 α-鼠李糖苷酶 柚苷酶 高效液相色谱法 

分 类 号：TQ925[轻工技术与工程—发酵工程]