高效液相色谱法检测中国传统发酵豆腐制品中的生物胺  

Determination of Biogenic Amines in Chinese Traditional Fermented Tofu by a High Performance Liquid Chromatography Method

作　　者：刘振锋[1] 魏云潇[1] 张进杰[1] 孙玉敬[1] 刘东红[1] 叶兴乾[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310029

出　　处：《中国食品学报》2010年第4期253-259,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金："十一五"国家科技支撑计划项目(2009BAD9B05)

摘　　要：目的:建立同时检测中国传统发酵豆制品中10种生物胺(BAs)的高效液相色谱法,并测定一些发酵豆腐制品中的生物胺含量。方法:以庚胺为内标试剂,苯甲酰氯为样品衍生试剂;Inertsil ODS-3色谱柱为固定相,乙腈和10mmol/L乙酸铵溶液为流动相,采用梯度洗脱,于波长254nm处紫外检测。以0.4mol/L高氯酸溶液为样品提取溶剂,测定发酵豆腐制品中10种生物胺含量。结果:在20min内各种生物胺得到很好的分离。经方法学验证,线性关系良好,回收率在94.7%～109.8%之间,精密度、重复性RSD均小于10%。4种发酵豆腐制品中,被检测到的生物胺有:腐胺(Put)、尸胺(Cad)、亚精胺(Spd)、精胺(Spm)、色胺(Try)、2-苯乙胺(Phe)、组胺(His)、酪胺(Tyr)。不同样品的生物胺含量差异很大,在所有样品中均未检出5-羟基色胺(Ser)、胍基丁胺(Agm)。结论:该方法灵敏度高,重现性好,是测定发酵豆腐制品中生物胺含量的有效方法。Objective：A HPLC method for qualitative and quantitative of ten biogenic amines（BAs） in Chinese traditional fermented tofu was studied,and the contents of biogenic amines in fermented tofu were determined.Method：Heptamine was used as the internal standard;Derivatisation of the samples was carried out using benzyl chloride;Inertsil ODS-3 column was used as the stationary phase and a gradient elution system was used for analysis with a mixture of acetonitrile and 10 mmol/L ammonium acetate solutions,and UV detector was applied at 254 nm.Biogenic amines were extracted by 0.4 mol/L HClO4 solution and then ten biogenic amines were measured simultaneously in fermented tofu.Results：The method had a good resolution in 20 minutes and good linearity,the recovery rate was between 94.7%～ 109.8%,and the RSD of precision,repeatability were all lower than 10%.Putrescine（Put）,cadverine（Cad）,spermidine（Spd）,spemine（Spm）,histamine（His）,tryptamine（Try）,2-phenethyleamine（Phe） and tyramine（Tyr） were detected in four kinds of fermented tofu,the content of biogenic amines had substantial variation in various samples,and agmatine（Agm）,serotonin（Ser） were not detected in all samples.Conclusion：With the merits of high sensitivity,precision and well repeatability,the method was feasible for the determination of biogenic amines in fermented tofu,and the biogenic amines forming factors need further study in Chinese traditional fermented tofu.

关 键 词：腐乳 臭豆腐干 生物胺 高效液相色谱法 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]