纳豆菌糖苷酶水解大豆异黄酮的条件优化  

Optimal hydrolysis of soybean isoflavone with glycosidase from Bacillus natto

作　　者：芦明春[1] 马素果[1] 杨红[1] 王应男[1] 罗平[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2010年第5期342-345,共4页Journal of Dalian Polytechnic University

基　　金：辽宁省教育厅创新团队项目(2008T009)

摘　　要：为得到生物活性较高的大豆异黄酮苷元,研究了纳豆菌所产糖苷酶水解大豆异黄酮的条件。通过薄层层析(TLC)检测,选择单因素条件。在单因素试验的基础上,进行四因素三水平的正交试验。结果表明,最佳水解条件为:水解温度40℃,水解时间3 h,底物质量浓度2 mg/mL,酶底体积比2∶1,在此条件下,水解率为84.65%。经HPLC检测,与染料木素标准品谱图对照,发现在糖苷酶水解作用下,大豆异黄酮糖苷很大程度上转化为苷元,且染料木素的含量显著增加。The hydrolysis of soybean isoflavone with glycosidase from Bacillus natto was studied. The condition of single-factor experiment was chosen by TLC. Orthogonal test was carried out based on single-factor experiment. The results showed that the optimal hrdrolysis were hydrolysis temperature 40 ℃, hydrolysis time 3 h, substrate concentration 2 mg/mL, enzyme-substrate ratio 2 - 1. The hydrolysis rate was 84.65% at the optimal conditions. It was found that soybean isoflavone glycosides were converted to isoflavone alycones with glycosidase. Genistein increased significantly

关 键 词：纳豆菌 糖苷酶 大豆异黄酮 

分 类 号：TS201.25[轻工技术与工程—食品科学]