乳酸乳杆菌胞外多糖的发酵条件优化  

Optimization of Fermentation Conditions for Exopolysaccharid Production from Lactobacillus lactis

作　　者：陈若雯[1] 李里[1] 段家彩[1] 卢琪[1] 高丽[1] 潘思轶[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品与生物技术学报》2010年第5期770-776,共7页Journal of Food Science and Biotechnology

基　　金：湖北省农业科技创新团队项目(2007-620)

摘　　要：对乳酸乳杆菌发酵生产胞外多糖的发酵条件进行了优化。在确定Elliker培养基为最适培养基的基础上,利用Plackett-Burman试验设计,筛选出对胞外多糖产量有显著影响的因素,即酵母粉、发酵时间、初始pH值,然后针对这3个关键因子,用单因子试验及中心组合试验优化得到最佳发酵条件。结果表明:当酵母粉为17.35 g/L,初始pH 5.85,发酵时间23.18 h时,乳酸乳杆菌胞外多糖实际产量可达3.910 g/L。This study aims to increase exopolysaccharid production d by optimizing the culture and nutrient conditions of Lactobacillus lactis.The results showed that Elliker was the optimum medium.Three statistically significant ingredients,yeast extract,the initial pH and fermentation time,which were determined by Plackett-Burman design.Based on the results of the first phase,the five-level three-factor central composite design was employed to optimize the above critical factors.The exopolysaccharid titer was achieved at 3.910 g/L,with the combination of yeast extract 17.35 g/L,the initial pH 5.85,fermentation time 23.18 h.

关 键 词：乳酸乳杆菌 胞外多糖 PLACKETT-BURMAN设计 响应曲面法 中心组合设计 

分 类 号：R151.2[医药卫生—营养与食品卫生学]