邻二氮菲-Fe^(2+)法测定保健食品的抗氧化能力  

Determination of Antioxidant Capacity of Healthcare Foods by Phenanthroline-Fe^(2+) Method

作　　者：刘薇[1] 王宏君[1] 赵建[1] 文悦[1] 文镜[1] 

机构地区：[1]生物活性物质与功能食品北京市重点实验室,北京联合大学应用文理学院,北京100191

出　　处：《食品科学》2010年第18期333-337,共5页Food Science

基　　金：北京市教委科技发展计划项目(KM201011417006);北京市教委重点实验室引导基金项目(21202543503)

摘　　要：目的:用邻二氮菲-Fe2+法测定保健食品抗氧化能力。方法:以Trolox为标准参照物,通过单因素分析,确定试剂用量及反应时间对线性关系、最低检测限、平行性精密度、重复性精密度、加标回收率等指标进行测定。结果:Trolox在质量浓度0.01～0.15g/L范围内线性关系良好;最低检测下限为0.0050g/L;平行性精密度为(375±12)μmol/g,变异系数3.2%;重复性精密度为(365±16)μmol/g,变异系数4.4%;5次重复加标回收率为(98.0±2.4)%。结论:改良的邻二氮菲-Fe2+法适用于保健食品抗氧化能力的检测。Purpose：To develop a phenanthroline-Fe2＋ method for determining the antioxidant capacity of healthcare foods.Methods：Trolox was used as the reference substance for antioxidant capacity assessment.Some key determination conditions such as hydrogen peroxide amount,color development time,solvent type,etc were optimized.Method figures of merits including linearity,limit of detection,precision,repeatability and spike recovery were investigated.Results：The developed method presented good linearity when trolox concentration varied from 0.01 to 0.15g/L,with a limit of detection of 0.0050 g/L,a parallel precision（375 ± 12） μmol/g（3.2% variation coefficient）,a repeated precision of（365 ± 16） μmol/g（4.4% variation coefficient）,and an average spike recovery of（98.0 ± 2.4）%（n = 5）.Conclusions：This method is applicable to the determination of antioxidant capacity of healthcare foods.

关 键 词：保健食品 抗氧化能力 邻二氮菲-Fe2+法 

分 类 号：TS207.3[轻工技术与工程—食品科学]