蛹虫草深层培养联产纤溶酶和菌丝体的比拟放大研究  

Scale-up of fermentation of fibrinolytic enzyme and mycelia by Cordyceps militaris

作　　者：郭建华[1] 陈慧鑫[1] 刘晓兰[1] 高红[1] 邓永平[1] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院,齐齐哈尔161006

出　　处：《中国酿造》2010年第11期76-79,共4页China Brewing

基　　金：黑龙江省科技厅科技攻关计划项目(GB08B401-04);黑龙江省教育厅海外学人项目(1151hz021)

摘　　要：以溶氧速率常数KLa相等为放大原则,研究了从摇瓶培养至10L和100L发酵罐培养的规律,试验结果表明:在10L发酵罐中搅拌转速为50r/min且通风量为0.7m3/h时、搅拌转速为100r/min且通风量为0.6m3/h时、搅拌转速150r/min且通风量0.5m3/h时的KLa值与摇瓶优化条件下的KLa值一致;在100L发酵罐中搅拌转速为100r/min且通风量为3m3/h时、搅拌转速为150r/min且通风量为2m3/h时的KLa值与摇瓶优化条件下的KLa值一致。发酵试验验证在10L发酵罐中,搅拌转速为100r/min,通风量为0.6m3/h时发酵结果与摇瓶优化条件下的发酵结果一致。在100L发酵罐中,当搅拌转速为150r/min,通风量为2m3/h时,菌丝体产量达到了摇瓶优化结果。Based on the similarity of KLa, the scale-up fermentation were investigated from the shaking flasks to 10L and 100L ferrnentor in this paper. Experimental results showed that, in 10L fermentor, the KLa under 50 r/min agitation speed and 0.7m3/h aeration, 100r/min and 0.6m3/h and t 50r/min and 0.5m3/h were the same to the KLa of shaking flask. In 100L fermentor, the KLa under 100r/min agitation speed and 3 m3/h aeration and 150r/min and 2m3/h were the same to the KLa of the shaking flask. In 10L fermentor, when agitation speed was set 100r/min and aeration was 0.6m3/h, the fermentation result was the same to that in shaking flasks. In the 100L fermentor, when agitation speed was 150r/min and aeration was 2m3/h, the yield ofmycelia was the same to that in shaking flasks.

关 键 词：蛹虫草 纤溶酶 菌丝体 比拟放大 发酵罐 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]